Müller D G, Schmid C E
Fakultät für Biologie der Universität Konstanz.
Biol Chem Hoppe Seyler. 1988 Aug;369(8):647-53. doi: 10.1515/bchm3.1988.369.2.647.
Pheromone secretion in living female gametes of Ectocarpus siliculosus was examined by closed-loop extraction and gas chromatography. The pheromone (ectocarpene) recovery efficiency was calibrated with synthetic ectocarpene and was found to depend strongly on the volume of the closed-loop system. Maximum extraction efficiency of ectocarpene (17.4%) was obtained with a small-volume system. In addition to the main compound ectocarpene, the pheromone bouquet of E. siliculosus contained minor amounts of hormosirene, multifidene and dictyotene, which until now are known only as sperm-attractants in other brown algae. The pheromone bouquet was identical in 4 clones of different geographic origins. Settled female gametes of Ectocarpus siliculosus continued to secrete pheromone and to attract male gametes for up to 7 h after their release with an ectocarpene secretion rate of 1 x 10(5) molecules s-1 cell-1.
通过闭环萃取和气相色谱法检测了绳藻活雌配子中的信息素分泌情况。用合成的ectocarpene对信息素（ectocarpene）的回收效率进行了校准，发现其强烈依赖于闭环系统的体积。在小体积系统中获得了ectocarpene的最大萃取效率（17.4%）。除了主要化合物ectocarpene外，绳藻的信息素组合还含有少量的hormosirene、multifidene和dictyotene，到目前为止，这些物质仅在其他褐藻中作为精子引诱剂为人所知。在4个不同地理来源的克隆中，信息素组合是相同的。绳藻的定居雌配子在释放后长达7小时内持续分泌信息素并吸引雄配子，ectocarpene的分泌速率为1×10⁵分子·秒⁻¹·细胞⁻¹。
