硒化壳聚糖通过 Fas/FasL 途径诱导肺癌细胞系 SPC-A-1 凋亡。
Seleno-Chitosan induces apoptosis of lung cancer cell line SPC-A-1 via Fas/FasL pathway.
机构信息
State Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China; QingYunTang Biotech (Beijing) Co., Ltd., No. 14, Zhonghe Street, Beijing Economic-Technological Development Area, Beijing 100176, China.
State Key Laboratory of Food Nutrition and Safety, Ministry of Education, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, China.
出版信息
Bioorg Chem. 2020 Apr;97:103701. doi: 10.1016/j.bioorg.2020.103701. Epub 2020 Feb 29.
In this study, human lung cancer SPC-A-1 cells were cultured with Seleno-Chitosan to study the mechanism of apoptosis. CCK-8 results showed that with the increase of the concentration of Seleno-Chitosan and the prolongation of culture time, the inhibition on the proliferation of SPC-A-1 cells gradually increased, and the morphology of SPC-A-1 cells changed obviously. Flow cytometry result suggested that the concentration of calcium ion, level of reactive oxygen species and mitochondrial membrane potential increased. Western blot showed that Seleno-Chitosan induced apoptosis via the mitochondrial pathway in SPC-A-1. Eventually, we found 15 proteins that were expressed abnormally by Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS), which were all related to Fas/FasL pathway of cells.
在这项研究中，用人肺癌 SPC-A-1 细胞与硒壳聚糖共培养，研究细胞凋亡的机制。CCK-8 结果表明，随着硒壳聚糖浓度的增加和培养时间的延长，对 SPC-A-1 细胞增殖的抑制作用逐渐增强，SPC-A-1 细胞的形态明显改变。流式细胞术结果提示钙离子浓度、活性氧水平和线粒体膜电位升高。Western blot 表明硒壳聚糖通过 SPC-A-1 细胞的线粒体途径诱导细胞凋亡。最终，我们通过基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）发现了 15 种异常表达的蛋白质，这些蛋白质均与细胞的 Fas/FasL 通路有关。
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