Wrocław University of Environmental and Life Sciences, Department of Biotechnology and Food Microbiology, Chełmońskiego 37, 51-630, Wrocław, Poland.
Wroclaw University of Science and Technology, Faculty of Environmental Engineering, Wybrzeże Wyspiańskiego 27, 50-370, Wrocław, Poland.
Environ Pollut. 2020 Jul;262:114281. doi: 10.1016/j.envpol.2020.114281. Epub 2020 Feb 27.
Recently it was demonstrated that mealworm (Tenebrio molitor) larvae consume and biodegrade polystyrene. Thus, in this study a breeding investigation with various types of polystyrene was performed to follow the changes in the gut microbiome diversity. Polystyrene used for packaging purposes (PSp) and expanded polystyrene (EPS) were perceived as more favorable and attacked more frequently by mealworms compared to raw polystyrene (PS) and material commercially available for parcels (PSp). Although our studies showed that larvae could bite and chew selected materials, they are not able to degrade and use them for consumption purposes. In a next-generation sequencing experiment, among all samples, seven classes, Gammaproteobacteria, Bacilli, Clostridia, Acidobacteria, Actinobacteria, Alphaproteobacteria and Flavobacteria, were indicated as the most abundant, whereas the predominant genera were Enterobacter, Lactococcus and Enterococcus. Additionally, we isolated three bacteria strains able to use diverse types of bioplastic as a sole carbon source. The strains with biodegradable activity against bioplastic were identified as species of the genera Klebsiella, Pseudomonas and Serratia. The presence of a bacterial strain able to degrade bioplastic may suggest a potential niche for further investigations.
最近的研究表明，黄粉虫幼虫能够食用和生物降解聚苯乙烯。因此，在这项研究中，我们对各种类型的聚苯乙烯进行了养殖调查，以跟踪肠道微生物多样性的变化。与原始聚苯乙烯（PS）和商业上用于包裹的材料（PSp）相比，用于包装的聚苯乙烯（PSp）和膨胀聚苯乙烯（EPS）被认为更受黄粉虫的欢迎，并且更频繁地被它们攻击。尽管我们的研究表明幼虫能够咬碎和咀嚼选定的材料，但它们无法降解并将其用于食用。在下一代测序实验中，在所有样本中，七个类别，γ-变形菌纲、芽孢杆菌、梭菌、酸杆菌、放线菌、α-变形菌纲和黄杆菌，被指示为最丰富的，而主要的属是肠杆菌、乳球菌和肠球菌。此外，我们分离出了三种能够将多种类型的生物塑料用作唯一碳源的细菌菌株。对生物塑料具有可生物降解活性的菌株被鉴定为克雷伯氏菌、假单胞菌和沙雷氏菌属的物种。能够降解生物塑料的细菌菌株的存在可能表明进一步研究的潜在生态位。
