Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Research Center of Environmental Diagnosis and Contamination Remediation, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
Key Laboratory of Pollution Processes and Environmental Criteria (Ministry of Education), Tianjin Engineering Research Center of Environmental Diagnosis and Contamination Remediation, College of Environmental Science and Engineering, Nankai University, Tianjin 300350, China.
Sci Total Environ. 2020 Jun 10;720:137582. doi: 10.1016/j.scitotenv.2020.137582. Epub 2020 Feb 25.
The toxic effects of multi-nanomaterial systems are receiving increasing attention owing to their inevitable release of various nanomaterials. Knowledge of the bioavailability of the new carbon material ball-milled biochar (BMB) and its synergistic toxicity with metal oxide nanoparticles in bacteria is currently limited. In this study, the interactions of BMB with copper oxide nanoparticles (CuO NPs) and their synergistic toxicity towards Streptomyces coelicolor M145 were analyzed. Results showed that the cytotoxicity, ROS level and permeability of cells changed greatly with the pyrolysis temperatures of biochar and the concentrations of CuO NPs. The greatest cytotoxicity (up to 63.1%) was achieved by adding 20 mg/L CuO NPs to BMB700. The ROS level and cell permeability of this treatment was also the highest, about 4.2 folds and 2.9 folds greater than that of control, respectively. The combination of 10 mg/L BMB700 with 10 mg/L CuO NPs can maximize production of antibiotics, with the yield of undecylprodigiosin (RED) and actinorhodin (ACT) 3.0 times and 4.2 times higher than that in the control, respectively, and the change trend of related genes was consistent with that of antibiotics production. Mechanism analysis showed that the different adsorption capacity of BMB of different pyrolysis temperatures on copper ions played a vital role in the synergistic toxicity, and the increase in cell membrane permeability caused by cell collisions with particles was also an important reason for cytotoxicity. Overall, the synergistic toxicity of BMB with other NPs varies the pyrolysis temperatures, when considering the synergistic toxicity of these materials, the preparation conditions need to be taken into account so as to assess their environmental risks more accurately. On the other hand, this research may provide a new approach for the antibiotic industry to increase its output.
多纳米材料系统的毒性效应因其各种纳米材料的不可避免释放而受到越来越多的关注。目前，人们对新的碳材料球磨生物炭（BMB）的生物可利用性及其与金属氧化物纳米颗粒在细菌中的协同毒性知之甚少。在这项研究中，分析了 BMB 与氧化铜纳米颗粒（CuO NPs）的相互作用及其对链霉菌（Streptomyces coelicolor M145）的协同毒性。结果表明，细胞的细胞毒性、ROS 水平和通透性随生物炭的热解温度和 CuO NPs 的浓度而发生很大变化。添加 20 mg/L CuO NPs 到 BMB700 时，细胞毒性最大（高达 63.1%）。该处理的 ROS 水平和细胞通透性也最高，分别比对照高约 4.2 倍和 2.9 倍。10 mg/L BMB700 与 10 mg/L CuO NPs 的组合可以最大限度地提高抗生素的产量，其中深红紫素（RED）和放线菌素（ACT）的产量分别比对照高 3.0 倍和 4.2 倍，相关基因的变化趋势与抗生素产量一致。机制分析表明，不同热解温度的 BMB 对铜离子的不同吸附能力对协同毒性起着至关重要的作用，颗粒与细胞碰撞引起的细胞膜通透性增加也是细胞毒性的重要原因。总的来说，BMB 与其他 NPs 的协同毒性因热解温度而异，在考虑这些材料的协同毒性时，需要考虑其制备条件，以便更准确地评估其环境风险。另一方面，这项研究可能为抗生素工业提供一种提高产量的新方法。
