Department of Molecular Neurobiology, Institute for Developmental Research, Aichi Developmental Disability Center, 713-8 Kamiya, Kasugai, Aichi, 480-0392, Japan.
Department of Neurochemistry, Nagoya University Graduate School of Medicine, Nagoya, Japan.
Med Mol Morphol. 2020 Dec;53(4):221-228. doi: 10.1007/s00795-020-00248-4. Epub 2020 Mar 7.
Septins are a highly conserved family of GTPases which are identified in diverse organisms ranging from yeast to humans. In mammals, nervous tissues abundantly contain septins and associations of septins with neurological disorders such as Alzheimer's disease and Parkinson's disease have been reported. However, roles of septins in the brain development have not been fully understood. In this study, we produced a specific antibody against mouse SEPT1 and carried out biochemical and morphological characterization of SEPT1. When the expression profile of SEPT1 during mouse brain development was analyzed by western blotting, we found that SEPT1 expression began to increase after birth and the increase continued until postnatal day 22. Subcellular fractionation of mouse brain and subsequent western blot analysis revealed the distribution of SEPT1 in synaptic fractions. Immunofluorescent analyses showed the localization of SEPT1 at synapses in primary cultured mouse hippocampal neurons. We also found the distribution of SEPT1 at synapses in mouse brain by immunohistochemistry. These results suggest that SEPT1 participates in various synaptic events such as the signaling, the neurotransmitter release, and the synapse formation/maintenance.
Septins 是一种高度保守的 GTPases 家族，存在于从酵母到人类等各种生物体中。在哺乳动物中，神经组织中富含 septins，并且已经报道了 septins 与神经退行性疾病（如阿尔茨海默病和帕金森病）的关联。然而，septin 在大脑发育中的作用尚未完全阐明。在这项研究中，我们制备了针对小鼠 SEPT1 的特异性抗体，并对 SEPT1 进行了生化和形态学特征分析。通过 Western blot 分析 SEPT1 在小鼠大脑发育过程中的表达谱，我们发现 SEPT1 的表达在出生后开始增加，并持续增加到出生后第 22 天。对小鼠大脑的亚细胞分离和随后的 Western blot 分析显示 SEPT1 分布在突触部分。免疫荧光分析显示 SEPT1 在原代培养的小鼠海马神经元突触处的定位。我们还通过免疫组织化学发现 SEPT1 在小鼠大脑中的突触处分布。这些结果表明 SEPT1 参与了各种突触事件，如信号转导、神经递质释放和突触形成/维持。
