Department of Basic Medical Sciences, Physiology Section, The University of the West Indies, Kingston, Jamaica.
Bio-Tech R&D Institute, Kingston, Jamaica.
Fitoterapia. 2020 Jun;143:104539. doi: 10.1016/j.fitote.2020.104539. Epub 2020 Mar 5.
Cannabis is one of the most chemically complex plants containing a large number of compounds, among which are cannabinoids; the main biologically active constituents of the plant. Delta-9-tetrahydrocannabinol (THC) is known as the main psychoactive cannabinoid. Studies have shown that the effects of THC can be modulated by cannabidiol (CBD), another major cannabinoid which has several beneficial effects of its own. This study aims to look at the effect of different concentrations of THC and CBD separately and in combination on the release of oxygen from erythrocytes by measuring the p50 of the oxygen haemoglobin dissociation curve. Blood samples were collected from twenty four healthy adult male and female non-smokers. P50 values were measured using the Hemox Analyzer for different concentrations (0, 2.5, 25, 50 and 100 ng/ml) of THC and CBD respectively, as well as with combinations of THC and CBD in 4:1 and 1:1 ratios. The results indicate that there is a decrease in P with increasing concentrations of both THC and CBD separately and in combination. The decrease in P was significant (p < .05) at all concentrations of THC and CBD. The changes observed with increasing concentrations of both THC and CBD suggests that they have an increased affinity for oxygen. This may have implications for individuals who consistently use cannabis.
大麻是化学结构最复杂的植物之一，含有大量化合物，其中包括大麻素；这是植物中主要的生物活性成分。Δ-9-四氢大麻酚（THC）是已知的主要精神活性大麻素。研究表明，大麻素的作用可以通过大麻二酚（CBD）来调节，CBD 是另一种主要的大麻素，它本身具有多种有益作用。本研究旨在分别观察不同浓度的 THC 和 CBD 以及它们的组合对红细胞释放氧气的影响，方法是测量氧合血红蛋白解离曲线的 p50。从 24 名健康的成年男女非吸烟者中采集血样。使用 Hemox 分析仪测量不同浓度（0、2.5、25、50 和 100ng/ml）的 THC 和 CBD 以及 THC 和 CBD 以 4:1 和 1:1 比例组合的 p50 值。结果表明，随着 THC 和 CBD 浓度的增加，p50 值均呈下降趋势，而且这种下降在所有 THC 和 CBD 浓度下均具有统计学意义（p<0.05）。随着 THC 和 CBD 浓度的增加而观察到的变化表明，它们对氧气的亲和力增加。这可能对经常使用大麻的人产生影响。
