Amiri Ghazale, Abbasi Shaye Maryam, Bahreini Masoumeh, Mafinezhad Asghar, Ghazvini Kiarash, Sharifmoghadam Mohammad Reza
Deparment of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, Iran.
Department of Microbiology, Shahid Kamyab Hospital, Mashhad University of Medical Sciences, Mashhad, Iran.
Iran J Microbiol. 2019 Oct;11(5):368-372.
In recent years, reports of strains resistant to all known antibiotics have caused a great concern in medical communities. Overexpression of efflux pumps is one of the major causes of resistance in bacteria. The aim of this study was to investigate the role of efflux pumps in conferring resistance to imipenem in clinically important and .
A total number of 46 clinical isolates, including 33 and 13 isolates, previously collected from Shahid Kamyab and Ghaem hospitals of Mashhad, Iran were used in this study. Imipenem susceptibility testing was carried out by the disc diffusion method. Imipenem minimum inhibitory concentration (MIC) for resistant isolates were determined both in the presence and absence of the efflux pumps inhibitor, carbonyl cyanide 3-chlorophenylhydrazone (CCCP).
Resistance to imipenem was observed in 38 isolates including 30 and 8 isolates. Experiments in the presence of CCCP showed a 2 to 16384 fold reduction in imipenem MICs in 14 and 2 isolates.
The results obtained showed high levels of resistance to imipenem and contribution of efflux pumps in conferring resistance in both species in this study. Moreover, imipenem efflux mediated resistance highlights the importance of this mechanism not only in but also in non- Spp. which have been neglected in antibiotic resistance studies.
近年来，对所有已知抗生素均耐药的菌株报告在医学界引起了极大关注。外排泵的过度表达是细菌耐药的主要原因之一。本研究的目的是调查外排泵在临床上重要的[细菌名称1]和[细菌名称2]对亚胺培南耐药中的作用。
本研究使用了总共46株临床分离株，包括先前从伊朗马什哈德的沙希德·卡米亚布医院和加姆医院收集的33株[细菌名称1]分离株和13株[细菌名称2]分离株。采用纸片扩散法进行亚胺培南敏感性试验。在有和没有外排泵抑制剂羰基氰3-氯苯腙（CCCP）的情况下，测定耐药[细菌名称1]分离株的亚胺培南最低抑菌浓度（MIC）。
在38株分离株中观察到对亚胺培南耐药，包括30株[细菌名称1]和8株[细菌名称2]分离株。在CCCP存在的情况下进行的实验表明，14株[细菌名称1]和2株[细菌名称2]分离株的亚胺培南MIC降低了2至16384倍。
本研究结果表明，在本研究的两种[细菌名称]中，对亚胺培南的耐药水平较高，且外排泵在赋予耐药性方面发挥了作用。此外，亚胺培南外排介导的耐药性突出了这一机制不仅在[细菌名称1]中，而且在抗生素耐药性研究中被忽视的非[细菌名称1]物种中的重要性。
