School of Science, Jiangnan University, Wuxi 214122, China.
Biomed Res Int. 2020 Feb 18;2020:5189549. doi: 10.1155/2020/5189549. eCollection 2020.
The incidence of chronic myeloid leukemia (CML) is increasing year by year, which is a serious threat to human health. Early diagnosis can reduce mortality and improve prognosis. LncRNAs have been shown to be effective biomarkers for a variety of diseases and can act as competitive endogenous RNA (ceRNA). In this study, the dysregulated lncRNA-associated ceRNA networks (DLCN) of the chronic phase (CP), accelerated phase (AP), and blastic crisis (BC) for CML are constructed. Then, based on dynamic network biomarkers (DNB), some dysregulated lncRNA-associated ceRNA network biomarkers of CP, AP, and BC for CML are identified according to DNB criteria. Thus, a lncRNA (SNHG5) is identified from DLCN_CP, a lncRNA (DLEU2) is identified from DLCN_AP, and two lncRNAs (SNHG3, SNHG5) are identified from DLCN_BC. In addition, the critical index (CI) used to detect disease outbreaks shows that CML occurs in CP, which is consistent with clinical medicine. By analyzing the functions of the identified ceRNA network biomarkers, it has been found that they are effective lncRNA biomarkers directly or indirectly related to CML. The result of this study will help deepen the understanding of CML pathology from the perspective of ceRNA and help discover the effective biomarkers of CP, AP, and BC for CML in the future, so as to help patients get timely treatment and reduce the mortality of CML.
慢性髓系白血病（CML）的发病率逐年增加，严重威胁着人类健康。早期诊断可以降低死亡率，改善预后。lncRNAs 已被证明是多种疾病的有效生物标志物，并可以作为竞争性内源 RNA（ceRNA）发挥作用。在本研究中，构建了 CML 慢性期（CP）、加速期（AP）和急变期（BC）的失调 lncRNA 相关 ceRNA 网络（DLCN）。然后，根据动态网络生物标志物（DNB）标准，从 CP、AP 和 BC 的 DNB 中鉴定出一些失调的 lncRNA 相关 ceRNA 网络生物标志物。因此，从 DLCN_CP 中鉴定出一个 lncRNA（SNHG5），从 DLCN_AP 中鉴定出一个 lncRNA（DLEU2），从 DLCN_BC 中鉴定出两个 lncRNAs（SNHG3，SNHG5）。此外，用于检测疾病爆发的关键指标（CI）表明 CML 发生在 CP，这与临床医学一致。通过分析鉴定的 ceRNA 网络生物标志物的功能，发现它们是直接或间接与 CML 相关的有效的 lncRNA 生物标志物。本研究的结果将有助于从 ceRNA 的角度加深对 CML 病理的理解，并有助于未来发现 CML 的 CP、AP 和 BC 的有效生物标志物，从而帮助患者得到及时治疗，降低 CML 的死亡率。
