rhBNP 对大鼠心脏骤停后心肌功能障碍的保护作用。

The Protective Effect of rhBNP on Postresuscitation Myocardial Dysfunction in a Rat Cardiac Arrest Model.

机构信息

Department of Basic and Clinical Pharmacology, School of Pharmacy, Anhui Medical University, Hefei, China.

Department of Intensive Care Unit, The Second Affiliated Hospital of Anhui Medical University, Hefei, China.

出版信息

Biomed Res Int. 2020 Feb 17;2020:6969053. doi: 10.1155/2020/6969053. eCollection 2020.

PURPOSE

We investigated the protective effects and the underlying mechanisms through which recombinant human brain natriuretic peptide (rhBNP) acts on postresuscitation myocardial dysfunction (PRMD) in the cardiac arrest (CA) model.

METHODS

Ventricular fibrillation was induced and untreated for 6 min. And the time of cardiopulmonary resuscitation was 8 min, after which defibrillation was attempted in this rat model. 24 Sprague Dawley rats (450-550g) were randomized into cardiopulmonary resuscitation (CPR) + rhBNP and CPR + placebo groups after restoration of spontaneous circulation (ROSC). rhBNP was infused at PR 30 min (loading dose: 1.5 g/kg, 3 min; maintenance dose: 0.01 g/kg, 3 min; maintenance dose: 0.01 (TNF- (TNF- (TNF-B (NF-B (NF.

RESULTS

The administration of rhBNP attenuated the severity of PRMD and myocardial tissue injuries, with improvement of MAP (mean arterial blood pressure), ETCO (end-tidal CO), serum level of NT-proBNP, EF, CO, and MPI values. The serum levels and protein expression levels in myocardial tissue of IL-6 and TNF- (TNF-B (NF.

CONCLUSION

Our research demonstrated that the administration of rhBNP attenuated the severity of PRMD and myocardial tissue injuries and increased the 24 h survival rate in this CA model. rhBNP administration also reduced the serum and myocardial tissue levels of IL-6 and TNF- after ROSC, likely due to the suppression of the TLR4/NF-B signaling pathway and the regulation of inflammatory mediator secretion. (TNF-B (NF.

目的

本研究旨在探讨重组人脑利钠肽（rhBNP）对心脏骤停（CA）模型再灌注后心肌功能障碍（PRMD）的保护作用及其作用机制。

方法

诱导心室颤动，不予治疗 6 分钟。心肺复苏（CPR）时间为 8 分钟，然后尝试对该大鼠模型进行除颤。24 只 Sprague Dawley 大鼠（450-550g）在自主循环恢复（ROSC）后随机分为 CPR+rhBNP 和 CPR+安慰剂组。rhBNP 在 PR 后 30 分钟内输注（负荷剂量：1.5μg/kg，3 分钟；维持剂量：0.01μg/kg，3 分钟；维持剂量：0.01μg/kg/min，持续 180 分钟）。