Rucklidge G J, Riddoch G I, Williams L M, Robins S P
Rowett Research Institute, Bucksburn, Aberdeen, Scotland.
Coll Relat Res. 1988 Jul;8(4):339-48. doi: 10.1016/s0174-173x(88)80005-0.
Cyanogen bromide (CNBr) peptides of [3H]-labelled type I collagen isolated from young rat skin were injected into adult rats via the tail vein. After 1, 5 and 10 minutes, the fate of the circulating radioactive peptides was investigated by measurement of the tissue distribution of radioactivity. Rapid removal of peptides from the circulation and their concomitant accumulation in the kidney proximal tubule epithelia was confirmed by autoradiography of kidney sections at the light microscope level. Immunopositive material was also identified and localized in proximal tubules using antibodies directed against denatured type I collagen chains and their CNBr degradation products. Ultrastructural autoradiography indicated that rapid removal of collagen peptides from the glomerular filtrate occurs via the brush border, apical vacuoles, and lysosomes.
从幼鼠皮肤中分离出的[3H]标记的I型胶原蛋白的溴化氰（CNBr）肽经尾静脉注射到成年大鼠体内。在1、5和10分钟后，通过测量放射性的组织分布来研究循环中放射性肽的命运。通过光学显微镜水平的肾脏切片放射自显影证实了肽从循环中快速清除以及它们在肾近端小管上皮中的伴随积累。使用针对变性I型胶原链及其CNBr降解产物的抗体，还在近端小管中鉴定并定位了免疫阳性物质。超微结构放射自显影表明，胶原蛋白肽从肾小球滤液中的快速清除是通过刷状缘、顶端空泡和溶酶体进行的。
