Institute of Pharmacy, I.M. Sechenov First Moscow State Medical University (Sechenov University), Moscow, Russia.
Faculty of Chemistry, M.V. Lomonosov Moscow State University, Moscow, Russia.
Phytochem Anal. 2020 Sep;31(5):662-669. doi: 10.1002/pca.2931. Epub 2020 Mar 9.
Aflatoxin B1 (AFB1) is a toxic low-molecular-weight secondary metabolite of Aspergillus flavus and A. parasiticus. AFB1 was classified as a Group I carcinogen by the World Health Organisation for Research on Cancer in 1993. AFB1 is an unavoidable natural contaminant of some herbal medicine, able to cause serious health issues for humans consuming the related medicine.
Therefore, this study aimed to develop an efficient fluorescence polarisation immunoassay (FPIA) and a rapid, low-cost, and easy-to-use membrane-based flow-through immunoassay (MBA) for determination of AFB1 in herbal medicine Origanum vulgare L., Rubus idaeus L., Urtica dioica L. and Sorbus aucuparia L.
A cut-off level of the developed MBA was 0.8 ppb. Validation of the developed test was performed with blank and spiked samples. Using three naturally contaminated or three artificially spiked samples. The FPIA showed a linear working range of 8.6 to 64 ppb, and a half maximal inhibitory concentration (IC ) of 24 ppb.
The results were in good correlation with the enzymelinked immunosorbent assay (ELISA) results (the IC 0.1 ppb). Both the sample preparation and analysis are simple, cost-effective and easy to perform on-site in non-laboratory environments. Liquid chromatography with tandem mass spectrometry (LC-MS/MS) was used as a confirmatory technique.
黄曲霉毒素 B1（AFB1）是黄曲霉和寄生曲霉的一种低分子量次级代谢物，具有毒性。1993 年，世界卫生组织下属的癌症研究机构将 AFB1 列为 I 类致癌物质。AFB1 是某些草药中不可避免的天然污染物，能对食用相关药物的人类健康造成严重问题。
因此，本研究旨在开发一种高效荧光偏振免疫分析（FPIA）和一种快速、低成本且易于使用的基于膜的流动免疫分析（MBA），用于测定草药牛至、覆盆子、荨麻和欧洲花楸中的 AFB1。
开发的 MBA 的截止值为 0.8 ppb。采用空白和加标样品对开发的试验进行了验证。使用三个自然污染的样品或三个人工加标的样品。FPIA 的线性工作范围为 8.6 至 64 ppb，半最大抑制浓度（IC ）为 24 ppb。
结果与酶联免疫吸附试验（ELISA）结果（IC 0.1 ppb）相关性良好。样品制备和分析都简单、具有成本效益，且易于在非实验室环境中现场进行。液相色谱-串联质谱法（LC-MS/MS）被用作确证技术。
