Institute of Problems of Chemical Physics, Russian Academy of Science, Chernogolovka, Moscow, Russia.
Scientific and Educational Center of the Moscow State Regional University in Chernogolovka, Mytishchi, Moscow, Russia.
Bull Exp Biol Med. 2020 Feb;168(4):583-588. doi: 10.1007/s10517-020-04757-1. Epub 2020 Mar 9.
We performed a comparative study of the cytotoxicity of cisplatin, JM216 complex, and aminonitroxyl platinum(IV) complexes for HeLa cells grown in monolayer and 3D culture. The growth dynamics of spheroids was studied and optimal conditions for evaluation of cytotoxicity were determined. Spheroids were less sensitive to the test compounds than cells in a monolayer. The resistance index (RI) of spheroids was determined as the ratio of IC for spheroids to IC for monolayer culture. Resistance index was 5.0±1.5 for cisplatin and ranged from 1.8 to 2.3 for platinum(IV) complexes. The observed differences are related to different physicochemical properties of the complexes and different mechanisms of their penetration into cells.
我们对顺铂、JM216 配合物和氨基亚硝酰基铂（IV）配合物在单层和 3D 培养的 HeLa 细胞中的细胞毒性进行了比较研究。研究了球体的生长动力学，并确定了评估细胞毒性的最佳条件。与单层培养的细胞相比，球体对测试化合物的敏感性较低。球体的耐药指数（RI）定义为球体的 IC 与单层培养的 IC 的比值。顺铂的耐药指数为 5.0±1.5，铂（IV）配合物的耐药指数范围为 1.8 至 2.3。观察到的差异与配合物的不同物理化学性质以及它们进入细胞的不同机制有关。
