Zhang Fan, Li Hao-Ying
Medical Research Council, Laboratory of Molecular Biology, Hills Road, Cambridge, UK.
Biomanufacturing Research Centre, School of Mechanical and Electronic Engineering, Soochow University, Suzhou, China.
Methods Mol Biol. 2020;2118:91-98. doi: 10.1007/978-1-0716-0319-2_6.
Therapeutic gene delivery systems offer the potential for the treatment of a range of inherited and acquired inherited diseases. In contrast with viral gene vectors, the nonviral gene vectors provide a safer alternative and additional advantages such as the improved delivery efficiency, low cost, and often unlimited capacity to package DNA. Here we describe the preparation of a nonviral gene delivery technique based on lipid-peptide-DNA (LPD) complexes. The size of LPD particles is in the nanometer range. The use of these nanoparticulate LPDs results in high efficiency transfections and a high level of gene expression in vitro. LPDs provide a convenient and efficient tool for gene delivery in gene therapy.
治疗性基因递送系统为一系列遗传性和获得性遗传病的治疗提供了可能性。与病毒基因载体相比，非病毒基因载体提供了一种更安全的选择以及其他优势，如提高递送效率、低成本以及通常无限的DNA包装能力。在此，我们描述了一种基于脂质 - 肽 - DNA（LPD）复合物的非病毒基因递送技术的制备方法。LPD颗粒的大小在纳米范围内。使用这些纳米级LPD可在体外实现高效转染和高水平的基因表达。LPD为基因治疗中的基因递送提供了一种方便且高效的工具。
