Department of Pharmaceutical Sciences, School of Pharmacy, Northeastern University, Boston, MA, USA.
Clinical Research Division, Fred Hutchinson Cancer Research Center, Seattle, WA, USA.
Methods Mol Biol. 2020;2118:99-110. doi: 10.1007/978-1-0716-0319-2_7.
Skewing the macrophage polarity to achieve a favorable phenotype is a recently investigated therapeutic strategy in multiple disease/dysfunctional conditions such as inflammation, tumors, autoimmune disorders, and tissue repairs. However, delivering the therapeutic agent specifically to the macrophages has been a challenge in this field. Here, we describe the synthesis of hyaluronic acid (HA)-based nanoparticles for targeting CD44 receptors on the macrophages. The HA backbone is modified with cationic polyethyleneimine (PEI) for efficient encapsulation of microRNA into the self-assembling nanoparticles for targeted delivery to macrophages.
将巨噬细胞极性向有利表型倾斜是近年来在多种疾病/功能障碍情况下（如炎症、肿瘤、自身免疫性疾病和组织修复）研究的一种治疗策略。然而，在该领域中，将治疗剂特异性递送至巨噬细胞一直是一个挑战。在这里，我们描述了基于透明质酸（HA）的纳米粒子的合成，用于靶向巨噬细胞上的 CD44 受体。HA 主链用阳离子聚乙烯亚胺（PEI）修饰，以将 microRNA 有效包封到自组装纳米颗粒中，从而靶向递送至巨噬细胞。
