Lin Fanli, Wang Shuyue, Xiong Hao, Liu Yang, Li Xiaoming, Huang Chunlan
Department of Hematology, Affiliated Hospital of Southwest Medical University, Luzhou, Sichuan, China.
The affiliated hospital of Panzhihua University, Panzhihua, Sichuan, China.
Biosci Rep. 2020 Mar 27;40(3). doi: 10.1042/BSR20192104.
The present study investigated the effects of microvascular endothelial cells (MECs) on the chemotaxis, adhesion and proliferation of bone marrow hematopoietic stem cells (HSCs) ex vivo.
MECs were collected from the lung tissue of C57BL/6 mice, and HSCs were isolated with immunomagnetic beads from bone marrow of GFP mice. MECs and HSCs were co-cultured with or without having direct cell-cell contact in Transwell device for the measurement of chemotaxis and adhesion of MECs to HSCs. Experimental results indicate that the penetration rate of HSCs from the Transwell upper chamber to lower chamber in 'co-culture' group was significantly higher than that of 'HSC single culture' group. Also, the HSCs in co-culture group were all adherent at 24 h, and the co-culture group with direct cell-cell contact had highest proliferation rate. The HSC number was positively correlated with vascular endothelial growth factor (VEGF) and stromal cell-derived factor-1 (SDF-1) levels in supernatants of the culture.
Our study reports that MECs enhance the chemotaxis, adhesion and proliferation of HSCs, which might be related to cytokines SDF-1 and VEGF secreted by MECs, and thus MECs enhance the HSC proliferation through cell-cell contact. The present study revealed the effect of MECs on HSCs, and provided a basis and direction for effective expansion of HSCs ex vivo.
本研究在体外研究了微血管内皮细胞（MECs）对骨髓造血干细胞（HSCs）趋化性、黏附及增殖的影响。
从C57BL/6小鼠肺组织中收集MECs，并用免疫磁珠从绿色荧光蛋白（GFP）小鼠骨髓中分离HSCs。将MECs和HSCs在Transwell装置中进行共培养，有无直接细胞间接触，以测量MECs对HSCs的趋化性和黏附性。实验结果表明，“共培养”组中HSCs从Transwell上室向下室的穿透率显著高于“HSC单培养”组。此外，共培养组中的HSCs在24小时时均发生黏附，且直接细胞间接触的共培养组增殖率最高。培养上清液中HSC数量与血管内皮生长因子（VEGF）和基质细胞衍生因子-1（SDF-1）水平呈正相关。
我们的研究报告称，MECs增强了HSCs的趋化性、黏附及增殖，这可能与MECs分泌的细胞因子SDF-1和VEGF有关，因此MECs通过细胞间接触增强了HSC增殖。本研究揭示了MECs对HSCs的影响，为体外有效扩增HSCs提供了依据和方向。
