基因组DNA中大片段DNA的酶促扩增与表征
Enzymatic amplification and characterization of large DNA fragments from genomic DNA.
作者信息
Keohavong P, Wang C C, Cha R S, Thilly W G
机构信息
MIT Center for Environmental Health Sciences, Massachusetts Institute of Technology, Cambridge 02139.
出版信息
Gene. 1988 Nov 15;71(1):211-6. doi: 10.1016/0378-1119(88)90094-7.
PMID:3215526
Abstract
Conditions for DNA amplification in vitro using modified T7 DNA polymerase have been devised to obtain 2000-bp DNA fragments of the HGPRT gene directly from human genomic DNA. The DNA obtained from a 1.2 x 10(5)-fold amplification has been used for direct sequencing.
摘要
已设计出使用修饰的T7 DNA聚合酶进行体外DNA扩增的条件，以便直接从人类基因组DNA中获得HGPRT基因的2000碱基对DNA片段。从1.2×10⁵倍扩增中获得的DNA已用于直接测序。
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