Food Processing, Vocational School, Mus Alparslan University, Mus, Turkey.
Plant Production and Technologies, Faculty of Applied Sciences, Mus Alparslan University, Mus, Turkey.
Int J Phytoremediation. 2020;22(4):420-426. doi: 10.1080/15226514.2019.1667953. Epub 2019 Sep 30.
The aim of this study is to determine the effect of GA on maize seedlings as well as its antioxidative effect under copper (Cu) stress. To that end, maize seedlings were exposed to 1.5 mM GA and 1 mM Cu solutions, and the seedlings were allocated to following experimental groups: 18-hr distilled water (DI) (Control), 6-hr GA + 12-hr DI (GA), 6-hr DI + 12-hr Cu (Cu), 6-hr GA + 12-hr Cu (GA + Cu), 12-hr Cu +6-hr GA (Cu + GA), and 18-hr GA and Cu mixture (GA = Cu). The experiment was designed by randomized block design with three repetitions. The results showed that GA alone and GA + Cu treatments significantly decreased membrane damage and HO formation compared to the other treatments. GA formation was determined to be highest in turn in treatments GA + Cu and GA alone. The proline content significantly decreased in treatments GA alone and GA + Cu. The highest catalase (CAT) activity was observed in GA alone application, whereas GA + Cu treatment led to increase in guaiacol peroxidase (GPX), superoxide dismutase (SOD), and ascorbate peroxidase (APX) activities. GA has a protective effect on chlorophyll contents and relative water content (RWC), but not on carotenoid levels under Cu stress. Overall, GA alone application or GA + Cu stress may prevent the formation of reactive oxygen species (ROS) due to chelation effect of GA.
本研究旨在确定 GA 对玉米幼苗的影响及其在铜（Cu）胁迫下的抗氧化作用。为此，将玉米幼苗暴露于 1.5mM GA 和 1mM Cu 溶液中，并将幼苗分配到以下实验组：18 小时蒸馏水（DI）（对照）、6 小时 GA + 12 小时 DI（GA）、6 小时 DI + 12 小时 Cu（Cu）、6 小时 GA + 12 小时 Cu（GA+Cu）、12 小时 Cu + 6 小时 GA（Cu+GA）和 18 小时 GA 和 Cu 混合物（GA=Cu）。该实验采用随机区组设计，重复三次。结果表明，与其他处理相比，GA 单独处理和 GA+Cu 处理显著降低了膜损伤和 HO 的形成。GA 形成在 GA+Cu 和 GA 单独处理中依次最高。脯氨酸含量在 GA 单独和 GA+Cu 处理中显著降低。GA 单独处理的 CAT 活性最高，而 GA+Cu 处理导致 GPX、SOD 和 APX 活性增加。GA 在 Cu 胁迫下对叶绿素含量和相对水含量（RWC）具有保护作用，但对类胡萝卜素水平没有影响。总的来说，GA 单独处理或 GA+Cu 胁迫可能通过 GA 的螯合作用防止活性氧（ROS）的形成。
