Department of Agricultural, Environmental and Food Sciences, University of Molise, 86100 Campobasso, Italy.
Department of Nutrition and Food Science, University of Granada, Campus of Cartuja, 18071 Granada, Spain.
Food Res Int. 2020 Apr;130:108970. doi: 10.1016/j.foodres.2019.108970. Epub 2019 Dec 31.
The aim of this study was to evaluate the content of free and bound phenols and alkylresorcinols in functional couscous enriched with barley coarse fraction obtained by air classification. Two different levels of enriched barley flour (20 and 30%) were used for the formulation of couscous and they were compared with a control couscous made with 100% of semolina. HPLC-ESI-TOF-MS was used to determine the phenolic composition in couscous samples. Total free phenolic compounds content in control couscous was 182 μg/100 g d.m. This amount increases significantly when coarse barley flour is added reaching value of 2273 μg/100 g d.m. and 2978 μg/100 g d.m. when 20 and 30% of barley coarse fraction was used, respectively. Moreover, the main free phenolic compounds in enriched barley samples are represented by flavan-3-ols. Bound phenols ranged from 5242 μg/100 g d.m. for control couscous to 27,092 μg/100 g d.m. for couscous with 30% of barley coarse fraction with a strong prevalence of phenolic acids. Finally, regarding alkylresorcinol compounds, they ranged from 1.01 mg/g d.m. for control couscous to 2.46 mg/g d.m. for couscous with 30% of barley coarse fraction. In conclusion, on the basis of obtained results, barley coarse fraction can be considered a good ingredient to develop functional couscous naturally enriched of phenolic compounds and alkylresorcinols.
本研究旨在评估通过空气分级获得的富含大麦粗粉的功能性古斯米中游离酚和烷基间苯二酚的含量。使用两种不同水平的富含大麦面粉（20%和 30%）来配制古斯米，并将其与用 100%粗粒小麦粉制成的对照古斯米进行比较。HPLC-ESI-TOF-MS 用于测定古斯米样品中的酚类成分。对照古斯米中的总游离酚含量为 182μg/100g d.m. 当添加粗大麦粉时，这一数量显著增加，当分别使用 20%和 30%的大麦粗粉时，达到 2273μg/100g d.m. 和 2978μg/100g d.m. 此外，富含大麦样品中的主要游离酚类化合物由黄烷-3-醇组成。结合酚的含量范围从对照古斯米的 5242μg/100g d.m. 到含 30%大麦粗粉的古斯米的 27092μg/100g d.m.，其中以酚酸为主。最后，关于烷基间苯二酚化合物，其含量范围从对照古斯米的 1.01mg/g d.m. 到含 30%大麦粗粉的古斯米的 2.46mg/g d.m. 综上所述，根据所得结果，大麦粗粉可被视为一种良好的成分，可用于开发天然富含酚类化合物和烷基间苯二酚的功能性古斯米。
