School of Food Science and Engineering, Hefei University of Technology, Hefei, 230009, China.
School of Chemistry and Molecular Engineering, Nanjing Tech University, Nanjing, 211816, China.
Mikrochim Acta. 2020 Mar 10;187(4):212. doi: 10.1007/s00604-020-4209-8.
A selective and sensitive fluorescence biosensor is described for determination of microRNA-167 using fluorescent resonant energy transfer (FRET) strategy. The FRET system comprises carbon dots (CDs, donor) labeled with probe DNA (pDNA) and polydopamine (PDA)-coated FeO nanoparticles (FeO@PDA NPs, acceptor). The CDs-pDNA can be absorbed onto the surface of FeO@PDA NPs because of the strong π interaction between pDNA and PDA. With the enhanced adsorption ability of FeO@PDA NPs by Ca, the fluorescence intensity of CDs at 445 nm (excitation at 360 nm) is quenched. In presence of microRNA-167, the hybridized complex of CDs-pDNA-microRNA-167 will be released from the surface of FeO@PDA NPs due to the weak π interaction of the complex and PDA. This results in the fluorescence recovery of CDs. By application of twice-magnetic separation, the biosensor shows a wide linear range of 0.5-100 nM to microRNA-167 with a 76 pM detection limit. The method was applied to the determination of microRNA-167 in samples of total microRNA extractions from A. thaliana seedlings, and the recoveries ranged from 96.4 to 98.3%.
一种用于测定 microRNA-167 的选择性和灵敏性荧光生物传感器，采用荧光共振能量转移（FRET）策略。FRET 系统由碳点（CDs，供体）标记的探针 DNA（pDNA）和聚多巴胺（PDA）包覆的 FeO 纳米粒子（FeO@PDA NPs，受体）组成。由于 pDNA 和 PDA 之间的强π相互作用，CDs-pDNA 可以被吸附到 FeO@PDA NPs 的表面。由于 Ca 的存在增强了 FeO@PDA NPs 的吸附能力，CDs 在 445nm 处的荧光强度（在 360nm 处激发）被猝灭。在存在 microRNA-167 的情况下，由于复合物和 PDA 之间的弱π相互作用，CDs-pDNA-microRNA-167 杂交复合物将从 FeO@PDA NPs 的表面释放。这导致 CDs 的荧光恢复。通过两次磁分离的应用，该生物传感器对 microRNA-167 显示出从 0.5-100nM 到 76pM 的检测限的宽线性范围。该方法应用于从拟南芥幼苗总 microRNA 提取物中测定 microRNA-167，回收率范围为 96.4-98.3%。
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