Clinical Laboratory Center, Gansu Provincial Maternity and Child care Hospital, Lanzhou, Gansu Province, China.
Department of Rehabilitation Medicine, Children's Hospital of Nanjing Medical University, Nanjing, China.
Cell Biochem Funct. 2020 Oct;38(7):955-964. doi: 10.1002/cbf.3521. Epub 2020 Mar 11.
Long non-coding RNA (lncRNA) LINC00899 is one kind cytoplasmic lncRNA, however, there is rarely little information about its function in physiological process. Here, we demonstrated that lncRNA LINC00899 was upregulated in acute myeloid leukaemia (AML) cells and was quite correlated with poor prognosis of AML patients. High expression of LINC00899 in AML cells could promote cell proliferation and inhibit cell apoptosis, and facilitate the progression of AML consequently both in vitro and in vivo. Besides, LINC00899 acted as a molecular sponge of miR-744-3p. Furthermore, we characterized YY1 as the direct target of miR-744-3p, and LINC00899/miR-744-3p interaction modulated YY1 expression in AML cells. Finally, we verified LINC00899 modulated AML cell proliferation and apoptosis via regulating YY1. Our study revealed novel mechanism about how did lncRNA LINC00899 execute function in AML and thus provided potential therapeutic interventions for AML. SIGNIFICANCE OF THE STUDY: LncRNA LINC00899 is upregulated in AML cells and is correlated with poor prognosis of AML patients. LncRNA LINC00899 mediates cell proliferation and apoptosis of acute myeloid leukaemia cells. Knockdown of LINC00899 inhibited the growth of xenograft glioma tumour in vivo. LINC00899 acts as a molecular sponge of miR-744-3p. YY1 is the downstream target of LINC00899/miR-744-3p signalling.
长链非编码 RNA（lncRNA）LINC00899 是一种细胞质 lncRNA，但关于其在生理过程中的功能却鲜有信息。在这里，我们证明 lncRNA LINC00899 在急性髓系白血病（AML）细胞中上调，并且与 AML 患者的不良预后密切相关。AML 细胞中 LINC00899 的高表达可促进细胞增殖并抑制细胞凋亡，从而在体外和体内促进 AML 的进展。此外，LINC00899 作为 miR-744-3p 的分子海绵发挥作用。此外，我们将 YY1 鉴定为 miR-744-3p 的直接靶标，并且 LINC00899/miR-744-3p 相互作用调节了 AML 细胞中的 YY1 表达。最后，我们验证了 LINC00899 通过调节 YY1 来调节 AML 细胞的增殖和凋亡。我们的研究揭示了 lncRNA LINC00899 在 AML 中执行功能的新机制，从而为 AML 提供了潜在的治疗干预措施。研究意义：LINC00899 在 AML 细胞中上调，与 AML 患者的不良预后相关。LINC00899 介导急性髓系白血病细胞的增殖和凋亡。敲低 LINC00899 抑制了体内异种移植胶质细胞瘤肿瘤的生长。LINC00899 作为 miR-744-3p 的分子海绵。YY1 是 LINC00899/miR-744-3p 信号的下游靶标。
