大鼠长期服用棉酚后血浆、睾丸网液和附睾尾液中棉酚含量的相关性。

The correlation between the gossypol contents in blood plasma, rete testis fluid, and cauda epididymal fluid following chronic treatment with gossypol in rats.

作者信息

Wang J M, Qiu J P, Wu X L, Zhang Z R, Shao Y, Cao R Q

机构信息

Department of Pharmacology, Nanjing Institute of Materia Medica, People's Republic of China.

出版信息

J Androl. 1988 Nov-Dec;9(6):397-402. doi: 10.1002/j.1939-4640.1988.tb01072.x.

DOI:10.1002/j.1939-4640.1988.tb01072.x

PMID:3215825

Abstract

Concentrations of gossypol in blood plasma, rete testis fluid, and fluid from the caudia epididymidis were measured simultaneously by high performance liquid chromatography in rats treated with gossypol (15 mg/kg daily for 3 weeks). Antispermatogenic effects were demonstrated by loss of sperm motility in the cauda epididymidis and structural changes in the testis. It was found in these treated rats that concentrations of gossypol were lower in rete testis fluid compared with blood plasma but increased significantly in fluid from the cauda epididymidis. The results indicate a restriction of the blood-testis barrier to gossypol and its local concentration in the epididymis after fluid resorption.

摘要

采用高效液相色谱法同时测定了给予棉酚（每日15mg/kg，连续3周）的大鼠血浆、睾丸网液和附睾尾部液中的棉酚浓度。附睾尾部精子活力丧失和睾丸结构改变表明了抗生精作用。在这些经处理的大鼠中发现，与血浆相比，睾丸网液中的棉酚浓度较低，但在附睾尾部液中显著升高。结果表明血睾屏障对棉酚有限制作用，且液体重吸收后棉酚在附睾中的局部浓度升高。