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测定. 的溶血活性。

Characterization of the hemolytic activity of .

机构信息

Shanghai Veterinary Research Institute, the Chinese Academy of Agricultural Sciences, 518 Ziyue Road, Shanghai 200241, PR China.

出版信息

Microbiology (Reading). 2020 May;166(5):436-439. doi: 10.1099/mic.0.000896.

Abstract

infection causes serious economic losses in the duck industry worldwide. Acute septicemia and high blood bacterial loading in infected ducks indicate that may be able to obtain iron and other nutrients by lysing duck erythrocytes to support its rapid growth and proliferation in the blood. However, so far, little is known about the hemolytic activity of to duck erythrocytes. In this study, 29 of 52 . strains showed hemolytic activity on duck blood agar, whereas all the tested dba (with hemolytic activity on duck blood agar) and dba strains created pores in the duck red blood cells, with 4.35-9.03% hemolytic activity in a liquid hemolysis assay after incubation for 24 h. The concentrated culture supernatants of all the tested strains and the extracted outer membrane proteins (OMPs) from dba strains showed hemolytic activity on duck blood agar. These results, together with the median lethal dose (LD50) of some dba and dba strains in ducklings, suggested that there was no direct relationship between the hemolytic capacity of on duck blood agar and its virulence.

摘要

感染会在全球范围内给养鸭业造成严重的经济损失。急性败血性败血症和感染鸭血液中细菌载量高表明,可能能够通过裂解鸭红细胞来获取铁和其他营养物质,以支持其在血液中的快速生长和增殖。然而,到目前为止,人们对的溶血活性知之甚少。在这项研究中,52 株中的 29 株在鸭血琼脂上表现出溶血活性,而所有测试的 dba(在鸭血琼脂上具有溶血活性)和 dba 菌株在孵育 24 小时后在鸭红细胞上形成孔,在液体溶血测定中具有 4.35-9.03%的溶血活性。所有测试的菌株的浓缩培养上清液和从 dba 菌株中提取的外膜蛋白 (OMP) 在鸭血琼脂上均显示出溶血活性。这些结果,以及一些 dba 和 dba 菌株在雏鸭中的半数致死剂量 (LD50) ,表明在鸭血琼脂上的溶血能力与毒力之间没有直接关系。

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Characterization of the hemolytic activity of .测定. 的溶血活性。
Microbiology (Reading). 2020 May;166(5):436-439. doi: 10.1099/mic.0.000896.

本文引用的文献

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OmpA is a virulence factor of Riemerella anatipestifer.OmpA 是鸭疫里默氏杆菌的一种毒力因子。
Vet Microbiol. 2011 Jun 2;150(3-4):278-83. doi: 10.1016/j.vetmic.2011.01.022. Epub 2011 Feb 1.

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