School of Biological Sciences, Georgia Institute of Technology, Atlanta, GA, United States of America.
Wallace H. Coulter Department of Biomedical Engineering, Georgia Institute of Technology and Emory University, Atlanta, Georgia, United States of America.
PLoS One. 2020 Mar 11;15(3):e0230241. doi: 10.1371/journal.pone.0230241. eCollection 2020.
To facilitate precise and convenient control of biological sample temperature, we developed a low-cost device that can be used independently or with any stereomicroscope. The purpose of the device is to control the thermal environment during experimental intervals in which a specimen must be manipulated outside of an incubator, e.g. for dissection or slide-mounting in preparation for imaging. Sample temperatures can be both cooled to below and heated to above room temperatures, and stably maintained at a precision of +/- 0.1˚C. To demonstrate the utility of this device, we report improved characterization of the penetrance of a short-acting temperature-sensitive allele in C. elegans embryos, and identification of the upper temperature threshold for embryonic viability for six Caenorhabditis species. By controlling the temperature environment even as a specimen is manipulated, this device offers consistency and flexibility, reduces environmental noise, and enables precision timing in experiments requiring temperature shifts.
为了精确、方便地控制生物样本的温度，我们开发了一种低成本的设备，它可以独立使用，也可以与任何立体显微镜一起使用。该设备的目的是在实验间隔期间控制热环境，在实验间隔期间，必须在培养箱外操作样本，例如进行解剖或载玻片安装，以便进行成像。样本温度可以被冷却到低于室温，也可以被加热到高于室温，并以 +/- 0.1°C 的精度稳定保持。为了展示该设备的实用性，我们报告了对秀丽隐杆线虫胚胎中短时效温度敏感等位基因的穿透率的改进表征，以及确定了六个秀丽隐杆线虫物种的胚胎生存上限温度。通过在操作样本的同时控制温度环境，该设备提供了一致性和灵活性，减少了环境噪声，并使需要温度变化的实验能够进行精确的定时。
