Neurosurgery Department, Xinxiang Central Hospital, Xinxiang, Henan Province 453000, People's Republic of China.
Color Ultrasonic Room, People's Hospital of Huixian, Xinxiang, Henan Province 453600, People's Republic of China.
Drug Des Devel Ther. 2020 Feb 26;14:855-866. doi: 10.2147/DDDT.S229607. eCollection 2020.
The present study aimed to assess the effect of curcumin (Cur) on carotid artery restenosis following carotid endarterectomy (CEA) and its associated mechanism in vivo and in vitro.
Ang II was used to induce excessive proliferation of rabbit aortic smooth muscle cells (CCC-SMC-1) in order to establish a hemadostenosis cell model. Similarly, the animal model of carotid artery restenosis was established by carotid artery gas drying injury combined with high-fat feed prior to CEA. CCC-SMC-1 cells and animals were treated by Cur and its effects on neointimal hyperplasia, inflammation and oxidative stress were detected and observed. The proteins that were associated with the Raf/MEK/ERK pathway were detected in cells and rabbit carotid artery tissues.
Cur inhibited the proliferation of smooth muscle cells and neointimal formation and reduced the inflammation and oxidative stress indices. Concomitantly, Cur reduced the phosphorylation of the Raf/MEK/ERK pathway proteins.
Cur could inhibit carotid restenosis following CEA by inhibiting the activation of the Raf/MEK/ERK pathway.
本研究旨在评估姜黄素（Cur）对颈动脉内膜切除术后颈动脉再狭窄的影响及其体内、体外的相关机制。
采用血管紧张素Ⅱ（AngⅡ）诱导兔主动脉平滑肌细胞（CCC-SMC-1）过度增殖，建立血管内膜增生细胞模型。同样，通过颈动脉气干伤联合高脂饲料喂养，在 CEA 前建立颈动脉再狭窄动物模型。用 Cur 处理 CCC-SMC-1 细胞和动物，检测和观察其对新生内膜增生、炎症和氧化应激的影响。检测细胞和兔颈动脉组织中与 Raf/MEK/ERK 通路相关的蛋白。
Cur 抑制了平滑肌细胞的增殖和新生内膜的形成，降低了炎症和氧化应激指标。同时，Cur 降低了 Raf/MEK/ERK 通路蛋白的磷酸化。
Cur 通过抑制 Raf/MEK/ERK 通路的激活，可抑制 CEA 后颈动脉再狭窄。
