College of Life Science and Technology, Lingnan Normal University, Zhanjiang, Guangdong, China.
Anim Biotechnol. 2021 Oct;32(5):602-609. doi: 10.1080/10495398.2020.1735406. Epub 2020 Mar 12.
In this study, embryos of from eye primordium formation to the larval growing stage were collected and used for RNA-Seq analysis. A total of 183,542,186 clean reads were assembled de novo into 58,054 unigenes consisting of 54,118,228 bp, with the average length at 932 bp and the N50 at 1667 bp. 21,469 (36.98%) unigenes were annotated at least in one of four databases including non-redundant protein (NR), Swiss-Prot, clusters of orthologous groups of proteins (KOG) and Kyoto Encyclopedia of Genes and Genomes (KEGG). 4460 (7.68%) unigenes were annotated in all databases. Analysis of differentially expressed genes (DEGs) was carried out on embryos at Eye primordium formation stage (SJ1), organ differentiation stage (SJ2), and hatching stage (SJ3). Overall, the current study provided the de novo assembly of transcriptome and identified the DEGs and pathways during embryonic development, which will provide a fundamental genetic resource for further functional research.
在这项研究中，我们收集了从眼原基形成到幼虫生长阶段的胚胎，并用于 RNA-Seq 分析。总共组装了 183,542,186 条清洁读数，将其组装成 58,054 个非冗余基因，包含 54,118,228 bp，平均长度为 932 bp，N50 为 1667 bp。21,469（36.98%）个基因至少在四个数据库中的一个数据库中进行了注释，包括非冗余蛋白（NR）、Swiss-Prot、蛋白质直系同源群（KOG）和基因和基因组百科全书（KEGG）。4460（7.68%）个基因在所有数据库中均有注释。对眼原基形成期（SJ1）、器官分化期（SJ2）和孵化期（SJ3）的胚胎进行差异表达基因（DEGs）分析。总的来说，本研究提供了转录组的从头组装，并鉴定了胚胎发育过程中的 DEGs 和途径，这将为进一步的功能研究提供基础遗传资源。
