Ministry of Education Key Laboratory of Analytical Science for Food Safety and Biology, College of Chemistry, Fuzhou University, Fuzhou, Fujian 350116, China.
State Key Laboratory of Environmental and Biological Analysis, Department of Chemistry, Hong Kong Baptist University, 224 Waterloo Road, Kowloon Tong, Hong Kong, SAR, P. R. China.
Analyst. 2020 Apr 14;145(8):3125-3130. doi: 10.1039/d0an00171f.
A spherical vinyl-functionalized covalent-organic framework (COF-V) was prepared at room temperature by a facile method and applied as a novel substrate for surface-enhanced laser desorption/ionization mass spectrometry (SELDI-MS). Compared with conventional organic matrices, the spherical COF-V with high crystallinity and good monodispersity exhibited high sensitivity, no matrix background interference, wide-range applicability, high salt tolerance and reproducibility in the characterization of small molecules. Considering these advantages, the applicability of the spherical COF-V-based SELDI-MS method was successfully demonstrated by determining trace amounts of glucose in diabetic urine, which would be a promising candidate for clinical diagnosis of diabetes. In addition, the morphological effect and the desorption/ionization mechanism of the COF-V were investigated in detail and the results indicated that the spherical COF-V substrate could greatly enhance the LDI process compared with the bulk COF-V. This work not only extends the application of COFs in MS, but also offers a promising alternative for small molecule identification and clinical diagnosis of diabetes.
一种球形的乙烯基功能化共价有机骨架（COF-V）通过简便的方法在室温下制备，并被用作表面增强激光解吸/电离质谱（SELDI-MS）的新型基质。与传统的有机基质相比，具有高结晶度和良好单分散性的球形 COF-V 表现出高灵敏度、无基质背景干扰、适用范围广、高耐盐性和在小分子表征中的重现性。考虑到这些优势，通过测定糖尿病尿液中的痕量葡萄糖，成功地证明了基于球形 COF-V 的 SELDI-MS 方法的适用性，这将是糖尿病临床诊断的有前途的候选者。此外，详细研究了 COF-V 的形态效应和解吸/电离机制，结果表明，与块状 COF-V 相比，球形 COF-V 基质可以大大增强 LDI 过程。这项工作不仅扩展了 COFs 在 MS 中的应用，而且为小分子鉴定和糖尿病的临床诊断提供了一种有前途的替代方法。
