Yi Xunfei, Lan Xiaopeng
Institute for Laboratory Medicine, 900 Hospital of the Joint Logistics Team, Navy Medical University (Second Military Medical University), Fuzhou, Fujian Province, China.
Biomed Chromatogr. 2020 Jul;34(7):e4826. doi: 10.1002/bmc.4826. Epub 2020 Mar 30.
Erianin is one of the bibenzyl ingredients isolated from Dctidrobium chrysotoxum Lindl. In recent years, erianin has attracted attention owing to its antitumor activity. In this study, an LC-MS/MS method was established to measure erianin in rat plasma. Gigantol was used as the internal standard. A Waters Acquity UPLC BEH C column was employed for chromatographic separation. The mobile phase consisted of water containing 0.1% formic acid and acetonitrile with a gradient elution at the flow rate of 0.4 ml/min. Selective reaction monitoring mode was used for quantitative analysis of erianin in positive electrospray ionization. In the concentration range of 0.1-1200 ng/ml, erianin in rat plasma was linear with correlation coefficient >0.999. The lowest limit of quantification was 0.1 ng/ml. The intra- and inter-day RSDs were <9.69%, while the RE was in the range of -8.59-11.24%. The mean recovery was >85.37%. Erianin was stable in rat plasma after storage under certain conditions. The validated method was demonstrated to be selective, sensitive and reliable, and has been successfully applied to pharmacokinetic study of erianin in rat plasma. Erianin was rapidly eliminated from rat plasma with a short half-life (〜1.5 h) and low oral bioavailability (8.7%).
毛兰素是从金石斛中分离得到的联苄类成分之一。近年来，毛兰素因其抗肿瘤活性而受到关注。本研究建立了一种LC-MS/MS方法来测定大鼠血浆中的毛兰素。吉兰泰被用作内标。采用Waters Acquity UPLC BEH C柱进行色谱分离。流动相由含0.1%甲酸的水和乙腈组成，以0.4 ml/min的流速进行梯度洗脱。采用选择性反应监测模式对正电喷雾电离中的毛兰素进行定量分析。在0.1-1200 ng/ml的浓度范围内，大鼠血浆中的毛兰素呈线性，相关系数>0.999。最低定量限为0.1 ng/ml。日内和日间相对标准偏差均<9.69%，相对误差在-8.59-11.24%范围内。平均回收率>85.37%。在一定条件下储存后，毛兰素在大鼠血浆中稳定。验证后的方法具有选择性、灵敏性和可靠性，并已成功应用于大鼠血浆中毛兰素的药代动力学研究。毛兰素从大鼠血浆中迅速消除，半衰期短（约1.5 h），口服生物利用度低（8.7%）。
