Alfano Flora, Fusco Giovanna, Mari Viviana, Occhiogrosso Leonardo, Miletti Gianluca, Brunetti Roberta, Galiero Giorgio, Desario Costantina, Cirilli Margie, Decaro Nicola
Istituto Zooprofilattico Sperimentale del Mezzogiorno, Portici (Napoli), Italy.
Dipartimento di Medicina Veterinaria, Università degli Studi di Bari, Valenzano (Bari), Italy.
Transbound Emerg Dis. 2020 Sep;67(5):1991-1999. doi: 10.1111/tbed.13542. Epub 2020 Apr 5.
Canine coronavirus (CCoV) strains with the ability to spread to internal organs, also known as pantropic CCoVs (pCCoVs), have been detected in domestic dogs and wild carnivores. Our study focused on the detection and molecular characterization of pCCoV strains circulating in Italy during the period 2014-2017 in autochthonous dogs, in dogs imported from eastern Europe or illegally imported from an unknown country. Samples from the gut and internal organs of 352 dogs were screened for CCoV; putative pCCoV strains, belonging to subtype CCoV-IIa, were identified in the internal organs of 35 of the examined dogs. Fifteen pCCoV strains were subjected to sequence and phylogenetic analyses, showing that three strains (98960-1/2016, 98960-3/2016, 98960-4/2016) did not cluster either with Italian or European CCoVs, being more closely related to alphacoronaviruses circulating in Asia with which they displayed a 94%-96% nucleotide identity in partial spike protein gene sequences. The pCCoV-positive samples were also tested for other canine viruses, showing co-infections mainly with canine parvovirus.
已在家犬和野生食肉动物中检测到具有传播至内部器官能力的犬冠状病毒（CCoV）毒株，即泛嗜性CCoV（pCCoV）。我们的研究聚焦于2014年至2017年期间在意大利本土犬、从东欧进口的犬或从未知国家非法进口的犬中传播的pCCoV毒株的检测和分子特征分析。对352只犬的肠道和内部器官样本进行了CCoV筛查；在35只受检犬的内部器官中鉴定出属于CCoV-IIa亚型的推定pCCoV毒株。对15株pCCoV毒株进行了序列和系统发育分析，结果显示，有三株毒株（98960-1/2016、98960-3/2016、98960-4/2016）既未与意大利或欧洲的CCoV聚类，而是与在亚洲传播的甲型冠状病毒关系更为密切，它们在部分刺突蛋白基因序列中与这些病毒的核苷酸同一性为94%-96%。对pCCoV阳性样本也检测了其他犬病毒，结果显示主要是与犬细小病毒的共感染。
