College of Sciences, China Agricultural University, Beijing, 100193, China.
State Key Laboratory of Environmental Criteria and Risk Assessment, Chinese Research Academy of Environmental Sciences, Beijing, 100012, China.
Environ Res. 2020 May;184:109310. doi: 10.1016/j.envres.2020.109310. Epub 2020 Feb 26.
The pesticide flutolanil has been detected in fish and aquatic environments, but its potential impact on the endocrine function is unknown. In this study, two-month zebrafish were exposed to the environmentally relevant concentrations of flutolanil for 60 days to examine the reproductive endpoints on the gonad endocrine system. Increased 17 beta-estradiol (17β-E2) content and 17β-E2/T ratio and decreased testosterone (T) in male suggested that flutolanil produces the estrogenic effect. In support of this view, vitellogene (vtg1, vtg2) and cytochrome P450 aromatase 19a (cyp19a) expression were up-regulated in the male liver. The levels of global DNA methylation were increased in ovary. Parental zebrafish exposure to different concentrations of flutolanil affected the offspring development as shown by short body length, and increased mortality. Thus, these results demonstrate that flutolanil exposure results in gonad endocrine disruption, decreased reproduction, and developmental toxicity in F1, highlighting the importance of assessing the potential environmental risks of flutolanil application.
农药氟虫腈已在鱼类和水生环境中被检测到，但它对内分泌功能的潜在影响尚不清楚。在这项研究中，将两个月大的斑马鱼暴露于环境相关浓度的氟虫腈中 60 天，以检查对性腺内分泌系统的生殖终点的影响。雄性鱼中 17β-雌二醇（17β-E2）含量和 17β-E2/T 比值增加，睾丸酮（T）减少，表明氟虫腈产生了雌激素效应。支持这一观点的是，雄性鱼肝脏中的卵黄原蛋白基因 1（vtg1、vtg2）和细胞色素 P450 芳香酶 19a（cyp19a）表达上调。卵巢中的总 DNA 甲基化水平升高。亲鱼暴露于不同浓度的氟虫腈会影响后代的发育，表现为体长缩短和死亡率增加。因此，这些结果表明，氟虫腈暴露会导致性腺内分泌紊乱，繁殖能力下降，并在 F1 代中产生发育毒性，突出了评估氟虫腈应用的潜在环境风险的重要性。
