Hess L, Chamberlin T, Ciment G
Department of Cell Biology and Anatomy, Oregon Health Sciences University, Portland 97201.
J Neurosci Res. 1988 Oct-Dec;21(2-4):101-6. doi: 10.1002/jnr.490210203.
In previous work, we found that the phorbol ester drug 12-O-tetradecanoyl phorbol acetate (TPA) reverses the developmental restriction of melanogenesis that occurs early in neural crest development, causing Schwann cell precursors to undergo a metaplastic transformation into melanocytes. In this study, we examine whether these effects of TPA may be mediated by changes in endogenous levels of protein kinase C (PKC) activities. We report that low levels of PKC activity are correlated with this adventitious pigmentation in the crest-derived cells of dorsal root ganglia both during normal development and following TPA treatment in culture. These results suggest that regulation of endogenous levels of PKC plays a role in developmental decisions that neural crest cells make during early embryogenesis.
在之前的研究中，我们发现佛波酯药物12-O-十四酰佛波醇-13-乙酸酯（TPA）可逆转神经嵴发育早期发生的黑色素生成的发育限制，导致雪旺细胞前体发生化生转变为黑素细胞。在本研究中，我们研究了TPA的这些作用是否可能由蛋白激酶C（PKC）活性的内源性水平变化介导。我们报告，在正常发育过程中以及培养中TPA处理后，背根神经节嵴衍生细胞中低水平的PKC活性与这种偶然的色素沉着相关。这些结果表明，PKC内源性水平的调节在神经嵴细胞在早期胚胎发生过程中做出的发育决定中起作用。
