College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, 410073, China.
College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, 410073, China.
Biochem Biophys Res Commun. 2020 May 14;525(4):823-829. doi: 10.1016/j.bbrc.2020.02.095. Epub 2020 Mar 9.
Barnacles are notorious marine fouling organisms. Their successful attachment to a substrate requires that they search for an appropriate habitat during their cyprid stage. A chemical cue called SIPC (Settlement-Inducing Protein Complex) has been shown to play a key role in the induction of cyprid gregarious settlement; however, the underlying biochemical mechanism remains unclear. Here, RNA-seq was used to examine the gene expression profiles of Amphibalanus amphitrite cyprids in response to SIPC and to identify SIPC-activated intracellular signaling pathways. A total of 389 unigenes were differentially expressed in response to SIPC, and cement protein genes were not among them. KEGG enrichment analysis suggested that SNARE interactions in the vesicular transport pathway were significantly influenced by SIPC treatment, indicating a possible role for SIPC in triggering protein transportation and secretion. Several genes with specific functions in metamorphosis were found among the differentially expressed genes (DEGs). GO (Gene Ontology) enrichment analysis revealed that the DEGs were significantly enriched in enamel mineralization pathways, suggesting that SIPC may also be involved in the activation of mineralization.
藤壶是著名的海洋污损生物。它们成功附着在基质上需要在幼体阶段寻找合适的栖息地。一种称为 SIPC（定居诱导蛋白复合物）的化学信号已被证明在诱导幼体群居定居中发挥关键作用；然而，其潜在的生化机制尚不清楚。在这里，使用 RNA-seq 技术来检测藤壶 Amphibalanus amphitrite 幼体对 SIPC 反应的基因表达谱，并鉴定 SIPC 激活的细胞内信号通路。总共 389 个基因在对 SIPC 的反应中表现出差异表达，其中不包括胶蛋白基因。KEGG 富集分析表明，SIPC 处理显著影响囊泡运输途径中的 SNARE 相互作用，表明 SIPC 可能在触发蛋白质运输和分泌中发挥作用。在差异表达基因（DEGs）中发现了一些在变态过程中具有特定功能的基因。GO（基因本体论）富集分析表明，DEGs 在牙釉质矿化途径中显著富集，表明 SIPC 也可能参与矿化的激活。
