Bashir Zafar Iqbal, Banks Paul James
School of Physiology, Pharmacology and Neuroscience, University of Bristol, Bristol, UK.
Brain Neurosci Adv. 2017 Sep 28;1:2398212817728229. doi: 10.1177/2398212817728229. eCollection 2017 Jan-Dec.
Some of the outstanding questions in neuroscience today are aimed at understanding the cellular and network mechanisms responsible for learned behaviours. Being able to identify and subsequently manipulate those specific neurones previously activated in a behavioural episode is key to this endeavour. A number of different methods have now been developed that enable this to be achieved. In this article, we highlight the Daun02-daunorubicin method of disrupting neuronal activity. Despite the fact that the Daun02-daunorubicin method has been used for a number of years and has been applied across a number of different experimental systems, the mechanism by which Daun02-daunorubicin disrupts neuronal activity is not clear. In this article, we summarise some of the advances that have been made by using this technology and we discuss potential mechanisms by which Daun02-daunorubicin disrupts neuronal function.
当今神经科学领域的一些突出问题旨在理解负责学习行为的细胞和网络机制。能够识别并随后操纵在行为事件中先前被激活的那些特定神经元是这一努力的关键。现在已经开发出多种不同的方法来实现这一点。在本文中，我们重点介绍了柔红霉素二聚体（Daun02）-柔红霉素破坏神经元活动的方法。尽管柔红霉素二聚体-柔红霉素方法已经使用多年，并已应用于许多不同的实验系统，但柔红霉素二聚体-柔红霉素破坏神经元活动的机制尚不清楚。在本文中，我们总结了使用该技术所取得的一些进展，并讨论了柔红霉素二聚体-柔红霉素破坏神经元功能的潜在机制。
