Matthews B G, Douglas H, Guiney D G
Department of Medicine, University of California, San Diego, Medical Center 92103.
Microb Pathog. 1988 Sep;5(3):207-13. doi: 10.1016/0882-4010(88)90023-x.
Eight strains of Plesiomonas shigelloides were assayed for enterotoxin production in the rabbit ileal loop assay. Seven strains required serial in vivo passage in the rabbit's intestine before enterotoxin activity was detected in the cells' filtrate. Enterotoxin production was readily lost with subculture of these toxinogenic cells. Heat treatment of the cells' filtrate from three strains that had never been passed in vivo led to detectable enterotoxin activity in three of six separate assays. Using LT, CT, STIa, STIb and STII as probes, no homology to the whole cell DNA of the eight strains was detected on Southern hybridization under low stringency conditions. The enterotoxin of P. shigelloides appears to be novel since production is induced by in vivo passage, activity is seen with heat treatment of cells' filtrate and there is no DNA homology to the cloned enterotoxin genes of Escherichia coli and Vibrio cholerae.
采用兔回肠袢试验对8株类志贺邻单胞菌进行肠毒素产生情况的检测。7株菌株需要在兔肠道内连续进行体内传代，才能在细胞滤液中检测到肠毒素活性。这些产毒素细胞经传代培养后，肠毒素的产生很容易丧失。对3株从未进行过体内传代的菌株的细胞滤液进行热处理，在6次独立试验中有3次检测到了可检测的肠毒素活性。以LT、CT、STIa、STIb和STII作为探针，在低严谨条件下进行Southern杂交时，未检测到与这8株菌株全细胞DNA的同源性。类志贺邻单胞菌的肠毒素似乎是新的，因为其产生是由体内传代诱导的，细胞滤液经热处理后有活性，并且与大肠杆菌和霍乱弧菌的克隆肠毒素基因没有DNA同源性。
