伴刀豆球蛋白A增强牙髓干细胞的增殖和成骨分化
Concanavalin A Enhanced Proliferation and Osteogenic Differentiation of Dental Pulp Stem Cells.
作者信息
Suardita Ketut, Arundina Ira, Tedjosasongko Udijanto, Yuliati Anita, Peeters Harry Huiz, Wijaksana I Komang Evan, Surboyo Meircurius Dwi Condro
机构信息
Department of Conservative Dentistry, Faculty of Dental Medicine, Universitas Airlangga, Jawa Timur, Indonesia.
Department of Oral Biology, Faculty of Dental Medicine, Universitas Airlangga, Jawa Timur, Indonesia.
出版信息
Eur J Dent. 2020 Feb;14(1):123-127. doi: 10.1055/s-0040-1702900. Epub 2020 Mar 13.
OBJECTIVE
Dental pulp stem cells (DPSCs) can be used as a component in the formation of regenerative dentine during direct pulp capping therapy. Concanavalin A (ConA) is a type of lectin with a molecular weight of 26 kDa derived from the plant. Lectins possess strong proliferation and differentiation abilities in various animal cells including lymphocytes, osteoblasts, and chondrocytes. The aim of study was to determine the effect of ConA on the proliferation and osteogenic differentiation of DPSCs .
MATERIALS AND METHODS
In this study, DPSCs were isolated from third molars before ConA induction was performed at concentrations of 5 and 10 μg/mL. The proliferation assay was determined by 3-(4,5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay. Osteogenic differentiation was determined by means of mineralization.
STATISTICAL ANALYSIS
Data were analyzed using analysis of variance and a Student's -test. The -value was set at 0.05.
RESULTS
The addition of 5 and 10 µg/mL of ConA to DPSCs can significantly increase the proliferation and osteogenic differentiation of DPSCs ( ≤0.05).
CONCLUSION
ConA can increase the proliferation and osteogenic differentiation of DPSCs.
目的
牙髓干细胞（DPSCs）可作为直接盖髓治疗中再生牙本质形成的一个组成部分。伴刀豆球蛋白A（ConA）是一种分子量为26 kDa的植物来源凝集素。凝集素在包括淋巴细胞、成骨细胞和软骨细胞在内的各种动物细胞中具有强大的增殖和分化能力。本研究的目的是确定ConA对DPSCs增殖和成骨分化的影响。
材料与方法
在本研究中，从第三磨牙中分离出DPSCs，然后分别用5 μg/mL和10 μg/mL的ConA进行诱导。通过3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）法测定细胞增殖情况。通过矿化作用来确定成骨分化情况。
统计分析
采用方差分析和学生t检验对数据进行分析。P值设定为0.05。
结果
向DPSCs中添加5 μg/mL和10 μg/mL的ConA可显著提高DPSCs的增殖和成骨分化能力（P≤0.05）。
结论
ConA可提高DPSCs的增殖和成骨分化能力。
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