Griffin Dale W, Banks Kenneth, Gregg Kurtis, Shedler Sarah, Walker Brian K
U.S. Geological Survey, 600 4th Street South, St. Petersburg, FL 33772, USA.
Broward County, Environmental Planning and Community Resilience Division, 115 South Andrews Avenue, Room 329-H, Fort Lauderdale, FL 33301, USA.
Antibiotics (Basel). 2020 Mar 11;9(3):118. doi: 10.3390/antibiotics9030118.
Water samples were collected at several wastewater treatment plants in southeast Florida, and water and sediment samples were collected along and around one outfall pipe, as well as along several transects extending both north and south of the respective outfall outlet. Two sets of samples were collected to address potential seasonal differences, including 38 in the wet season (June 2018) and 42 in the dry season (March 2019). Samples were screened for the presence/absence of 15 select antibiotic resistance gene targets using the polymerase chain reaction. A contrast between seasons was found, with a higher frequency of detections occurring in the wet season and fewer during the dry season. These data illustrate an anthropogenic influence on offshore microbial genetics and seasonal flux regarding associated health risks to recreational users and the regional ecosystem.
在佛罗里达州东南部的几个废水处理厂采集了水样，并沿着一条排污管及其周围，以及沿着分别位于排污口南北两侧延伸的几条样带采集了水和沉积物样本。为了研究潜在的季节差异，共采集了两组样本，其中雨季（2018年6月）采集了38份，旱季（2019年3月）采集了42份。使用聚合酶链反应对样本进行筛选，以检测15种选定的抗生素抗性基因靶点的存在与否。结果发现不同季节之间存在差异，雨季的检测频率较高，旱季较少。这些数据表明了人为因素对近海微生物遗传学的影响，以及与休闲使用者和区域生态系统相关健康风险的季节性变化。
