Department of Respiratory Diseases, Xi'an International Medical Center, Xi'an, Shanxi Province, China.
Department of Respiratory Diseases, Hefei Second People's Hospital, Hefei, Anhui Province, China.
Exp Lung Res. 2020 May-Jun;46(5):109-116. doi: 10.1080/01902148.2020.1725690. Epub 2020 Mar 13.
represents the most common opportunistic pathogen contributing to pneumonia in hospital-acquired infections. pneumonia has a rapidly progressive clinical course and multi-drug resistant (MDR). Identification of the effective biochemical markers is crucial for improving early diagnosis and treatment of pneumonia. The aims of our study are to 1) investigate the expression of β-Defensin-2(rβD2), IL-22, IL-22R1 and IL-10R2 in pneumonia-infected rats and 2) their association with the histological grades of pneumonia. Fifty specific pathogen free (SPF) male SD rats were randomly divided into two groups: control group (treated with normal saline) and pneumonia group (treated with ). All animals were sacrificed 1 h, 12 h, 1 d, 3 d, 5 d post infection. The severity and property of pneumonia was evaluated by histopathologic observation and pathogen identification. The mRNA expression of rβD2, IL-22, IL-22R1 and IL-10R2 was measured by RT-qPCR assay. The expression of rβD2 in rat lung tissue was determined by Western blot analysis, and the level of IL-22 in rat serum was determined by ELISA. Histopathologic examination and bacterial counting of lung tissues confirmed the successful establishment of rat pneumonia model. The gene expression of rβD2, IL-22, IL-22R1 and IL-10R2 in pneumonia rats were significantly higher than those in healthy control mice ( < 0.05). The expression of rβD2 was correlated with histological grades of pneumonia and the level of IL-22. RT-qPCR results showed that the peak expression of IL-22R1 appeared earlier than IL-10R2 in rat pneumonia model. The expression of rβD2 and IL-22 was increased significantly at early stage in rat pneumonia model, suggesting that IL-22 and rβD2 might serve as potential biomarkers for the early diagnosis of pneumonia.
代表最常见的机会性病原体，导致医院获得性感染的肺炎。肺炎具有快速进展的临床过程和多药耐药（MDR）。鉴定有效的生化标志物对于改善肺炎的早期诊断和治疗至关重要。我们的研究目的是 1）研究β-防御素-2（rβD2）、IL-22、IL-22R1 和 IL-10R2 在肺炎感染大鼠中的表达，2）它们与肺炎的组织学分级的关系。五十只特定病原体自由（SPF）雄性 SD 大鼠被随机分为两组：对照组（用生理盐水处理）和肺炎组（用处理）。所有动物在感染后 1 小时、12 小时、1 天、3 天和 5 天处死。通过组织病理学观察和病原体鉴定评估肺炎的严重程度和性质。通过 RT-qPCR 测定 rβD2、IL-22、IL-22R1 和 IL-10R2 的 mRNA 表达。通过 Western blot 分析测定大鼠肺组织中 rβD2 的表达，通过 ELISA 测定大鼠血清中 IL-22 的水平。肺组织的组织病理学检查和细菌计数证实了大鼠肺炎模型的成功建立。肺炎大鼠 rβD2、IL-22、IL-22R1 和 IL-10R2 的基因表达均明显高于健康对照组（<0.05）。rβD2 的表达与肺炎的组织学分级和 IL-22 的水平相关。RT-qPCR 结果表明，在大鼠肺炎模型中，IL-22R1 的表达峰值早于 IL-10R2。在大鼠肺炎模型的早期阶段，rβD2 和 IL-22 的表达明显增加，提示 IL-22 和 rβD2 可能作为肺炎早期诊断的潜在生物标志物。
