Department of Gastroenterology, The First Hospital of Hebei Medical Universit, China.
Department of Gastroenterology, Affiliated Hospital of Hebei University of Engineering, China.
J Coll Physicians Surg Pak. 2020 Mar;30(3):245-249. doi: 10.29271/jcpsp.2020.03.245.
To investigate the relationship between small intestinal bacterial overgrowth (SIBO) and Endotoxin (ET) concentration in peripheral blood, and levels of toll-like receptors (TLR) 2 and TLR4 expression on surface of peripheral blood mononuclear cells (PBMCs) in patients with ulcerative colitis.
An experimental study.
The First Hospital of Hebei Medical University, from July 2018 to October 2019.
The 130 patients with ulcerative colitis were included in case group. Another 72 healthy cases were selected as control group. SIBO, ET, TLR2, and TLR4, were determined, and compared.
Positive rate of SIBO in case group was higher than that in control group (p <0.001). Lactulose hydrogen breath test (LHBT) intestine set value, peripheral blood ET concentration, and TLR2 and TLR4 expression levels on surface of PBMCs in case group were higher than those in control group (all p <0.001); the above indexes in SIBO-positive patients in case group were higher than those in SIBO-negative patients in case group (all p <0.001). Pearson's correlation analysis showed that LHBT intestine set value of SIBO-positive patients in case group was positively correlated with ET concentration, and TLR2 and TLR4 expression levels on surface of PBMCs (r= 0.910, p <0.001; r = 0.970, p <0.001; and r = 0.965, p <0.001 respectively). ET concentration of SIBO-positive patients in case group was positively correlated with expression levels of TLR2 and TLR4 on surface of PBMCs (r=0.962, p <0.001; and r = 0.829 p <0.001 respectively).
Patients with ulcerative colitis are easy to occur SIBO, and SIBO increases blood endotoxin, TLR2 and TLR4 levels. Synergistic effects of endotoxin and endotoxin receptors TLR2 and TLR4 overexpression mediate body inflammation and may be involved in progression of ulcerative colitis. Patients with ulcerative colitis with excessive growth of small intestinal bacteria are more likely to have hypertoxemia.
研究小肠细菌过度生长（SIBO）与溃疡性结肠炎患者外周血内毒素（ET）浓度以及外周血单个核细胞（PBMC）表面 toll 样受体（TLR）2 和 TLR4 表达水平的关系。
实验研究。
河北医科大学第一医院，2018 年 7 月至 2019 年 10 月。
纳入 130 例溃疡性结肠炎患者为病例组，另选 72 例健康者为对照组。检测并比较两组 SIBO、ET、TLR2、TLR4。
病例组 SIBO 阳性率高于对照组（p<0.001）。病例组乳果糖氢呼气试验（LHBT）肠值、外周血 ET 浓度及 PBMC 表面 TLR2、TLR4 表达水平均高于对照组（均 p<0.001）；病例组 SIBO 阳性患者上述指标均高于病例组 SIBO 阴性患者（均 p<0.001）。Pearson 相关分析显示，病例组 SIBO 阳性患者 LHBT 肠值与 ET 浓度及 PBMC 表面 TLR2、TLR4 表达水平呈正相关（r=0.910，p<0.001；r=0.970，p<0.001；r=0.965，p<0.001）。病例组 SIBO 阳性患者 ET 浓度与 PBMC 表面 TLR2、TLR4 表达水平呈正相关（r=0.962，p<0.001；r=0.829，p<0.001）。
溃疡性结肠炎患者易发生 SIBO，SIBO 增加血内毒素、TLR2、TLR4 水平。内毒素及其受体 TLR2、TLR4 过度表达介导机体炎症反应，可能参与溃疡性结肠炎的进展。伴有小肠细菌过度生长的溃疡性结肠炎患者更易发生高血症。
