Husna Nazihah, Gascoigne Nicholas R J, Tey Hong Liang, Ng Lai Guan, Tan Yingrou
Singapore Immunology Network (SIgN), A*STAR (Agency for Science, Technology and Research), Biopolis, 8A Biomedical Grove, Singapore 138648, Singapore; Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore.
Department of Microbiology and Immunology, Yong Loo Lin School of Medicine, National University of Singapore, 5 Science Drive 2, Singapore 117545, Singapore.
Cell Immunol. 2020 Apr;350:104086. doi: 10.1016/j.cellimm.2020.104086. Epub 2020 Mar 10.
Optical imaging is a valuable tool to visualise biological processes in the context of the tissue. Each imaging modality provides the biologist with different types of information - cell dynamics and migration over time can be tracked with time-lapse imaging (e.g. intra-vital imaging); an overview of whole tissues can be acquired using optical clearing in conjunction with light sheet microscopy; finer details such as cellular morphology and fine nerve tortuosity can be imaged at higher resolution using the confocal microscope. Multi-modal imaging combined with image cytometry - a form of quantitative analysis of image datasets - provides an objective basis for comparing between sample groups. Here, we provide an overview of technical aspects to look out for in an image cytometry workflow, and discuss issues related to sample preparation, image post-processing and analysis for intra-vital and whole organ imaging.
光学成像在组织环境中可视化生物过程方面是一种有价值的工具。每种成像方式都为生物学家提供不同类型的信息——细胞动态和随时间的迁移可以通过延时成像（如活体成像）进行追踪；结合光片显微镜使用光学透明技术可以获取整个组织的概览；使用共聚焦显微镜可以以更高分辨率对细胞形态和精细神经曲折等更细微的细节进行成像。多模态成像与图像细胞术（一种对图像数据集进行定量分析的形式）相结合，为样本组之间的比较提供了客观依据。在此，我们概述了图像细胞术工作流程中需要注意的技术方面，并讨论了与活体和全器官成像的样本制备、图像后处理及分析相关的问题。
