Jeong Chang-Bum, Lee Bo-Young, Choi Beom-Soon, Kim Min-Sub, Park Jun Chul, Kim Duck-Hyun, Wang Minghua, Park Heum Gi, Lee Jae-Seong
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea; Department of Marine Science, College of Natural Sciences, Incheon National University, Incheon 22012, South Korea.
Department of Biological Science, College of Science, Sungkyunkwan University, Suwon 16419, South Korea.
Aquat Toxicol. 2020 May;222:105462. doi: 10.1016/j.aquatox.2020.105462. Epub 2020 Mar 2.
The copepod Tigriopus japonicus has been widely used as an experimental species in the field of ecotoxicology. We have sequenced and assembled the whole genome of T. japonicus with comparative analysis of gene families that represent detoxification phases in two additional public genomes of Tigriopus spp., namely, T. californicus and T. kingsejongensis. The total length of the T. japonicus assembled genome was 196.6 Mb with an N50 value of 10.65 Mb and consisted of 339 scaffolds and 25,143 annotated genes. The detoxification gene families encoding cytochrome P450s (CYP450s), glutathione S-transferases (GSTs), and ATP-binding cassette (ABC) proteins in Tigriopus spp. have shown species-dependent diversity in several gene sets, suggesting that these genes have undergone a species-specific expansion to increase their fitness to different marine habitats and environmental pressures. Our study will provide a better understanding of the detoxification system in Tigriopus spp. and will contribute to various areas of research, including ecotoxicology.
桡足类动物日本虎斑猛水蚤已在生态毒理学领域被广泛用作实验物种。我们对日本虎斑猛水蚤的全基因组进行了测序和组装，并与另外两个公共的虎斑猛水蚤属基因组（即加州虎斑猛水蚤和世宗大王虎斑猛水蚤）中代表解毒阶段的基因家族进行了比较分析。日本虎斑猛水蚤组装后的基因组全长为196.6兆碱基对，N50值为10.65兆碱基对，由339个支架和25143个注释基因组成。虎斑猛水蚤属中编码细胞色素P450（CYP450）、谷胱甘肽S-转移酶（GST）和ATP结合盒（ABC）蛋白的解毒基因家族在几个基因集中表现出物种依赖性多样性，这表明这些基因经历了物种特异性扩增，以提高它们对不同海洋栖息地和环境压力的适应性。我们的研究将有助于更好地理解虎斑猛水蚤属的解毒系统，并将为包括生态毒理学在内的各个研究领域做出贡献。
