Department of lipid chemistry, National Institute of Nutrition, Hyderabad, India.
Department of lipid chemistry, National Institute of Nutrition, Hyderabad, India.
Prostaglandins Leukot Essent Fatty Acids. 2020 Apr;155:102082. doi: 10.1016/j.plefa.2020.102082. Epub 2020 Feb 22.
In the present study, we investigated the impact of substituting alpha-linolenic acid (ALA) or long-chain n-3 PUFA (eicosapentaenoic acid and docosahexaenoic acid) for linoleic acid and hence decreasing n-6:n-3 PUFA ratio on high-fructose diet-induced hypertriglyceridemia and associated hepatic changes. Weanling male Wistar rats were divided into four groups and fed with starch-diet (n-6:n-3 PUFA ratio 215:1) and high-fructose diets with different n-6:n-3 PUFA ratio (215:1, 2:1 with ALA and 5:1 with long-chain n-3 PUFA) for twenty-four weeks. Substitution of linoleic acid with ALA (n-6:n-3 PUFA ratio of 2) or long-chain n-3 PUFA (n-6:n-3 PUFA ratio of 5) protected the rats from fructose-induced dyslipidemia, hepatic oxidative stress and corrected lipogenic and proinflammatory gene expression. Both ALA and long-chain n-3 PUFA supplementation also reversed the fructose-induced upregulation of 11β-hydroxysteroid dehydrogenase type 1 (11β-HSD1) gene, which is involved in the generation of active glucocorticoids in tissues. Although both ALA and LC n-3 PUFA prevented fructose-induced dyslipidemia to a similar extent, compared to ALA, LC n-3 PUFA is more effective in preventing hepatic oxidative stress and inflammation.
在本研究中，我们研究了用α-亚麻酸（ALA）或长链 n-3PUFA（二十碳五烯酸和二十二碳六烯酸）替代亚油酸，从而降低 n-6:n-3PUFA 比值对高果糖饮食诱导的高甘油三酯血症及相关肝变化的影响。将断乳雄性 Wistar 大鼠分为四组，分别用淀粉饮食（n-6:n-3PUFA 比值为 215:1）和不同 n-6:n-3PUFA 比值的高果糖饮食（215:1、ALA 替代的 2:1 和长链 n-3PUFA 替代的 5:1）喂养 24 周。用 ALA（n-6:n-3PUFA 比值为 2）或长链 n-3PUFA（n-6:n-3PUFA 比值为 5）替代亚油酸可保护大鼠免受果糖诱导的血脂异常、肝氧化应激，并纠正脂肪生成和促炎基因表达。ALA 和长链 n-3PUFA 的补充还逆转了果糖诱导的 11β-羟类固醇脱氢酶 1（11β-HSD1）基因的上调，该基因参与组织中活性糖皮质激素的产生。尽管 ALA 和 LC n-3PUFA 均可预防果糖诱导的血脂异常，但与 ALA 相比，LC n-3PUFA 更能有效预防肝氧化应激和炎症。
