Núcleo de Biotecnologia, Universidade Federal do Espírito Santo, Vitória, Espírito Santo, Brazil.
Instituto Capixaba de Pesquisa, Assistência Técnica e Extensão Rural, Vitória, Epírito Santo, Brazil.
Arch Virol. 2020 May;165(5):1211-1214. doi: 10.1007/s00705-020-04588-5. Epub 2020 Mar 13.
Papaya sticky disease (PSD), which can destroy orchards, was first attributed to papaya meleira virus (PMeV). However, the discovery of papaya meleira virus 2 (PMeV2) associated with PSD plants impose the need to detect this viral complex. We developed a multiplex RT-PCR (mPCR) technique capable of detecting two viruses in a single assay from pre-flowering plant samples, which is a useful tool for early diagnosis of PSD. We also determined the limit of detection (LOD) using asymmetric plasmid dilutions of both PMeV and PMeV2, which revealed that a higher titer of one virus prevents detection of the other. Thus, this technique is an alternative method for detecting PMeV and PMeV2 in a single reaction.
番木瓜粘性病（PSD）可摧毁果园，其最初被归因于番木瓜 ME 病毒（PMeV）。然而，与 PSD 植株相关的番木瓜 ME 病毒 2（PMeV2）的发现，使得检测这一病毒复合物成为必要。我们开发了一种多重 RT-PCR（mPCR）技术，能够从开花前的植株样本中同时检测两种病毒，这是 PSD 早期诊断的有用工具。我们还通过两种病毒的不对称质粒稀释来确定检测限（LOD），结果表明一种病毒的滴度较高会阻止对另一种病毒的检测。因此，该技术是在单次反应中同时检测两种病毒的替代方法。
