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具有 His 型亚铁氧化酶中心和外部金属结合位点的耐热 Dps 蛋白的生化和结构特征。

Biochemical and structural characterization of a thermostable Dps protein with His-type ferroxidase centers and outer metal-binding sites.

机构信息

Department of Chemistry and Biochemistry, Graduate School of Engineering, Kyushu University, Fukuoka, Japan.

International Institute for Carbon-Neutral Energy Research (WPI-I2CNER), Kyushu University, Fukuoka, Japan.

出版信息

FEBS Open Bio. 2020 Jul;10(7):1219-1229. doi: 10.1002/2211-5463.12837. Epub 2020 May 28.

DOI:10.1002/2211-5463.12837
PMID:32170832
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7327923/
Abstract

The DNA-binding protein from starved cells (Dps) is found in a wide range of microorganisms, and it has been well characterized. However, little is known about Dps proteins from nonheterocystous filamentous cyanobacteria. In this study, a Dps protein from the thermophilic nonheterocystous filamentous cyanobacterium Thermoleptolyngbya sp. O-77 (TlDps1) was purified and characterized. PAGE and CD analyses of TlDps1 demonstrated that it had higher thermostability than previously reported Dps proteins. X-ray crystallographic analysis revealed that TlDps1 possessed His-type ferroxidase centers within the cavity and unique metal-binding sites located on the surface of the protein, which presumably contributed to its exceedingly high thermostability.

摘要

饥饿细胞的 DNA 结合蛋白（Dps）存在于广泛的微生物中，并且已经得到了很好的描述。然而，对于非异形胞丝状蓝藻中的 Dps 蛋白知之甚少。在这项研究中，从嗜热非异形胞丝状蓝藻 Thermoleptolyngbya sp. O-77（TlDps1）中纯化并表征了一种 Dps 蛋白。PAGE 和 CD 分析表明，TlDps1 比以前报道的 Dps 蛋白具有更高的热稳定性。X 射线晶体学分析表明，TlDps1 在空腔内具有 His 型亚铁氧化酶中心和位于蛋白质表面的独特金属结合位点，这可能有助于其极高的热稳定性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/3135863f2a53/FEB4-10-1219-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/07c35c2cff35/FEB4-10-1219-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/6e71fb2f3e31/FEB4-10-1219-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/f2a7dfe68764/FEB4-10-1219-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/4a7b3fdceb0f/FEB4-10-1219-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/3135863f2a53/FEB4-10-1219-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/07c35c2cff35/FEB4-10-1219-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/6e71fb2f3e31/FEB4-10-1219-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/f2a7dfe68764/FEB4-10-1219-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/4a7b3fdceb0f/FEB4-10-1219-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ef89/7327923/3135863f2a53/FEB4-10-1219-g005.jpg

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