Heyworth M F, Kung J E, Caplin A B
Cell Biology Section, Veterans Administration Medical Center, San Francisco, California 94121.
Parasite Immunol. 1988 Nov;10(6):713-7. doi: 10.1111/j.1365-3024.1988.tb00257.x.
We describe an ELISA for trophozoite-specific IgA in the intestinal secretions of mice infected with Giardia muris. Using this method, trophozoite-specific IgA was demonstrated in intestinal secretions of Giardia-infected immunocompetent BALB/c mice. Such antibody was undetectable in the intestinal secretions of Giardia-infected athymic (nude) mice. Immunocompetent BALB/c mice are able to clear G. muris infection whereas nude mice are not. The study provides evidence that the chronicity of G. muris infection in nude mice results from lack of intestinal trophozoite-specific IgA in these animals. By means of the ELISA, trophozoite-specific IgA was demonstrated in intestinal secretions from immunocompetent mice in the absence of protease inhibitors.
我们描述了一种用于检测感染鼠贾第虫的小鼠肠道分泌物中滋养体特异性IgA的酶联免疫吸附测定（ELISA）方法。利用该方法，在感染贾第虫的具有免疫活性的BALB/c小鼠的肠道分泌物中证实了滋养体特异性IgA的存在。而在感染贾第虫的无胸腺（裸）小鼠的肠道分泌物中未检测到此类抗体。具有免疫活性的BALB/c小鼠能够清除鼠贾第虫感染，而裸小鼠则不能。该研究提供了证据，表明裸小鼠中鼠贾第虫感染的慢性化是由于这些动物缺乏肠道滋养体特异性IgA所致。通过ELISA方法，在没有蛋白酶抑制剂的情况下，在具有免疫活性的小鼠的肠道分泌物中证实了滋养体特异性IgA的存在。
