Gottstein B, Deplazes P, Tanner I
Institute of Parasitology, University of Berne, Switzerland.
Parasitol Res. 1993;79(8):644-8. doi: 10.1007/BF00932506.
Nu/+ mice (ZU.ICR-strain) experimentally infected with Giardia lamblia (clone GS/M-83-H7) cleared the infection by day 45 postinfection (p.i.). Athymic nu/nu mice were reconstituted with immune Peyer's patch lymphocytes obtained from self-healed nu/+ littermates and thus acquired the potential to decrease their intestinal parasite mass. Intestinal B-cells from self-healed nu/+ mice as well as from immune-reconstituted athymic nude mice synthesized in vitro parasite-specific immunoglobulin A (IgA). This IgA was subsequently analyzed by immunoblotting, showing a predominant reaction with the major surface antigen (a 72,000-Da polypeptide) characterizing the Giardia clone in question. The hypothesis on the causative role of intestinal IgA and immune lymphocytes in the control of G. lamblia infection thus deserves further attention.
用蓝氏贾第鞭毛虫（克隆株GS/M-83-H7）实验性感染的Nu/+小鼠（ZU.ICR品系）在感染后第45天清除了感染。无胸腺的nu/nu小鼠用从已自愈的nu/+同窝小鼠获得的免疫派尔集合淋巴结淋巴细胞进行重建，从而获得了降低其肠道寄生虫数量的潜力。来自已自愈的nu/+小鼠以及免疫重建的无胸腺裸鼠的肠道B细胞在体外合成了寄生虫特异性免疫球蛋白A（IgA）。随后通过免疫印迹分析这种IgA，结果显示其与所研究的贾第鞭毛虫克隆株的主要表面抗原（一种72,000道尔顿的多肽）有主要反应。因此，关于肠道IgA和免疫淋巴细胞在控制蓝氏贾第鞭毛虫感染中的致病作用的假说值得进一步关注。
