School of Chemical Sciences, Central University of Gujarat, Gandhinagar 382030, India.
School of Chemical Sciences, Central University of Gujarat, Gandhinagar 382030, India.
Int J Biol Macromol. 2020 Aug 1;156:1022-1044. doi: 10.1016/j.ijbiomac.2020.03.067. Epub 2020 May 15.
Lanthanide nanoparticles and nanorods especially their biocompatible oxide forms like cerium oxide nanoparticles (CNPs) with therapeutic applications are used to cure neurological oxidative stresses. Thus it tempts to study their biocompatible aspects by interactions with several biologically significant molecules. In-Vitro interactions of 15-240 μM CNPs with water, Phosphate buffered saline (PBS), DMEM media, Insulin (Ins) hemoglobin (Hb) and ds-DNA at 37 °C were studied. Their physicochemical properties study by Borosil Mansingh Survismeter (BMS) showed the first order interaction with the protein-protein structure breaking behaviour of CNPs with Hb, Ins, and DNA. Zeta potential measurements of CNPs in different biological medium show a net increase in negative charge magnitude with good colloidal stability. K = 4 × 106 mM of CNPs-DNA infer noncovalent interactions. Circular dichroism and FTIR revealed a loss of secondary conformation with increasing CNPs concentration. In-silico molecular docking depicts CNP interaction via conventional hydrogen bonding, carbon hydrogen bonding and electrostatic interactions at the minor groove of DNA. The study reports in-dept unfolding functional mechanism investigated by physicochemical, spectroscopic, and In-Silico approaches of protein on interactions with CNPs for safer-by-design use in medicine and pharmaceutics. Fundamentally the CeO in ~62% and CeO in ~38% with Ce and Ce oxidation potentials develop a unique case of electronic configurations with 4f5d6s and 4f5d6s electrons respectively which these studies a novel one.
镧系纳米粒子和纳米棒，特别是它们的生物相容性氧化物形式，如氧化铈纳米粒子（CNP），具有治疗应用，用于治疗神经氧化应激。因此，通过与几种具有生物学意义的分子相互作用来研究其生物相容性方面。在 37°C 下，研究了 15-240 μM 的 CNP 与水、磷酸盐缓冲盐水（PBS）、DMEM 培养基、胰岛素（Ins）、血红蛋白（Hb）和 ds-DNA 的体外相互作用。通过 Borosil Mansingh Survismeter（BMS）研究它们的物理化学性质表明，与 Hb、Ins 和 DNA 相互作用时，CNP 具有一级相互作用和蛋白质-蛋白质结构破坏行为。不同生物介质中 CNP 的 ζ 电位测量显示，随着带负电的 CNP 浓度的增加，其表面电荷的绝对值也在增加，具有良好的胶体稳定性。K = 4 × 106 mM 的 CNP-DNA 表明存在非共价相互作用。圆二色性和傅里叶变换红外光谱显示，随着 CNP 浓度的增加，二级构象丧失。分子对接表明 CNP 通过传统氢键、碳氢键和静电相互作用与 DNA 的小沟相互作用。该研究通过物理化学、光谱和计算机模拟方法，深入研究了蛋白质与 CNP 相互作用的展开功能机制，为在医学和药剂学中更安全地设计和使用 CNP 提供了依据。从根本上说，CeO 在 ~62%和 CeO 在 ~38%中，Ce 和 Ce 氧化还原电位分别具有独特的 4f5d6s 和 4f5d6s 电子组态，这些研究是新颖的。
