Biodiversity Research Center, Academia Sinica: 128, Academia Road, Section 2, Nankang, Taipei 11529, Taiwan; Institute of Ecology and Evolutionary Biology, National Taiwan University, No. 1, Sec. 4, Roosevelt Rd., Taipei 106, Taiwan.
Biodiversity Research Center, Academia Sinica: 128, Academia Road, Section 2, Nankang, Taipei 11529, Taiwan.
Ecotoxicol Environ Saf. 2020 May;194:110362. doi: 10.1016/j.ecoenv.2020.110362. Epub 2020 Mar 12.
The effects of microplastic on mortality and sublethal responses on larval development of meroplankton are still largely unknown. Present study investigated the effects of four sizes of virgin spherical polystyrene microplastics (diameter 1.7, 6.8, 10.4, 19.0 μm) on naupliar (stage II-VI) and cypris larvae of barnacle Amphibalanus amphitrite at environmentally relevant concentrations (1, 10, 100, 1000 beads mL). Essential life-history traits, including mortality, development time and rates of growth, settling, and metamorphosis were measured throughout the entire larval development. Feeding experiments were conducted to evaluate if microplastics decreased naupliar feeding due to physical impacts or selective feeding of nauplii. The results showed that A. amphitrite stage II nauplii were able to ingest and efficiently egest all sizes of microplastics. All the life-history endpoints measured were not significantly affected by all sizes of microplastics at any concentration tested. Presence of all sizes of microplastics did not cause physical interference on naupliar feeding and all stages of nauplius larvae (stage III-VI) did not selectively feed on microplastics. However, the feeding ability of stage III nauplius appeared to be affected by 1.7 μm at 1000 beads mL which was possibly due to individual variations rather than microplastics' impacts. Overall, the full larval development of barnacle A. amphitrite was not affected by microplastics at environmentally relevant concentrations under laboratory condition.
微塑料对浮游幼体死亡率和亚致死发育响应的影响仍知之甚少。本研究调查了四种尺寸（直径 1.7、6.8、10.4、19.0μm）的原始球形聚苯乙烯微塑料在环境相关浓度（1、10、100、1000 个珠/mL）下对藤壶 Amphibalanus amphitrite 无节幼体（Ⅱ-Ⅵ 期）和腺介幼虫的影响。整个幼体发育过程中测量了包括死亡率、发育时间和生长率、附着和变态在内的基本生活史特征。进行了摄食实验，以评估微塑料是否因物理影响或无节幼体的选择性摄食而降低无节幼体的摄食。结果表明，A. amphitrite Ⅱ期无节幼体能摄取并有效地排出所有尺寸的微塑料。在所有测试浓度下，所有尺寸的微塑料均未显著影响所有生活史终点。所有尺寸的微塑料的存在不会对无节幼体的摄食造成物理干扰，无节幼体的所有阶段（Ⅲ-Ⅵ 期）也不会选择性地摄食微塑料。然而，1000 个珠/mL 时 1.7μm 微塑料似乎影响了Ⅲ期无节幼体的摄食能力，这可能是由于个体差异而不是微塑料的影响。总体而言，在实验室条件下，环境相关浓度的微塑料不会影响藤壶 A. amphitrite 的完整幼体发育。
