Department of Clinical Microbiology, Faculty of Medicine and University Hospital, Charles University, Hradec Kralove, Czech Republic.
Department of Medical Microbiology, Charles University, 3rd Faculty of Medicine, Prague, Czech Republic.
Folia Microbiol (Praha). 2020 Jun;65(3):615-622. doi: 10.1007/s12223-020-00782-9. Epub 2020 Mar 14.
The aim of this study was to trace the dynamic changes of methicillin-resistant Staphylococcus aureus (MRSA) lineages in the local hospital in both the national and international context. We describe genotypic and phenotypic characterization of 62 non-duplicate MRSA isolates collected during 2010-2016 at University Hospital in Hradec Kralove, Czech Republic. The isolates were characterized by multilocus sequence typing (MLST), spa typing, and staphylococcal cassette chromosome mec typing (SCCmec typing). Eight different genotypes were described; ST225-t003-II (32/62, 52%), ST5-t002-II (13/62, 22%), and ST225-t014-II (12/62, 21%) were constantly detected over the 7-year follow-up period. The genotypes ST225-t151-II, ST225-t1282-II, ST225-t1623-II, ST78-t2832-II, and ST225-t8799-II occurred only once in the period reported. The majority of the strains, represented by ST225, belonged to clonal complex 5 (CC5).
本研究旨在追踪耐甲氧西林金黄色葡萄球菌(MRSA)在国内和国际背景下的医院内的动态变化。我们描述了 2010 年至 2016 年期间在捷克共和国赫拉德茨-克拉洛韦大学医院收集的 62 株非重复 MRSA 分离株的基因型和表型特征。这些分离株通过多位点序列分型(MLST)、spa 分型和葡萄球菌盒染色体 mec 分型(SCCmec 分型)进行了表征。描述了 8 种不同的基因型;ST225-t003-II(32/62,52%)、ST5-t002-II(13/62,22%)和 ST225-t014-II(12/62,21%)在 7 年的随访期间持续存在。基因型 ST225-t151-II、ST225-t1282-II、ST225-t1623-II、ST78-t2832-II 和 ST225-t8799-II 在报告期间仅出现过一次。代表 ST225 的大多数菌株属于克隆复合体 5(CC5)。