Pharmaceutical Informatics Institute, College of Pharmaceutical Sciences, Zhejiang University, Hangzhou 310058, China.
Key laboratory of Microbial Technology and Bioinformatics of Zhejiang Province, NMPA Key laboratory for Testing and Risk Warning of Pharmaceutical Microbiology, Zhejiang Institute of Microbiology, Hangzhou 310012, China.
Chin J Nat Med. 2020 Feb;18(2):90-102. doi: 10.1016/S1875-5364(20)30010-8.
With the occurrence of aging process, decreased neuron dopamine, disrupted brown adipose tissue (BAT) remodeling and decreased butyrate level all reflect a weak host healthy in certain degree. Nevertheless, the signs of mid-adult gut microbiota, and its association with host healthy are not well understood. In current study, we deemed to illustrate the associations of age, neuron dopamine, BAT remodeling, butyrate and gut microbiota with the aid of traditional herbal formula Kang Shuai Lao Pian (KSLP), which is known for its anti-aging effect. Here, ELISA was performed to detect the production of brain dopamine, the mass of inguinal white adipose tissue versus interscapular brown adipose tissue (iWAT/iBAT) was calculated and considered as a sign of BAT remodeling, 16S rRNA gene sequencing was used to the detection of gut microbiota profiling and gas chromatography was used to measure the butyrate level in mice feces. Our results indicated mid-adult mice already present distinctive gut microbiota profiling compared with young mice, concomitant with which are the lower brain dopamine level and disrupted brown adipose remodeling. KSLP treatment improved the host healthy and regulated gut microbiota with enriched Firmicutes at the expense of Bacteroidetes, particularly increased the relative abundance of bacteria functionally related to dopamine and butyrate productions, which suggest KSLP treatment constructs a healthier gut environment. In conclusion, modulation of gut microbiota and butyrate may connectively regulate dopamine production and BAT remodeling through gut-brain axis and gut-metabolism axis.
随着衰老过程的发生，神经元多巴胺减少、棕色脂肪组织（BAT）重塑紊乱以及丁酸盐水平降低都反映了宿主在一定程度上的健康状况下降。然而，中年期肠道微生物群的特征及其与宿主健康的关系尚不清楚。在本研究中，我们旨在借助具有抗衰老作用的传统中药方剂抗衰老片（KSLP）来阐明年龄、神经元多巴胺、BAT 重塑、丁酸盐和肠道微生物群与宿主健康之间的关联。在这里，我们通过酶联免疫吸附测定（ELISA）检测大脑多巴胺的产生，计算腹股沟白色脂肪组织与肩胛间棕色脂肪组织（iWAT/iBAT）的质量，并将其视为 BAT 重塑的标志，16S rRNA 基因测序用于检测肠道微生物群谱，气相色谱用于检测小鼠粪便中的丁酸盐水平。我们的研究结果表明，中年期小鼠与年轻小鼠相比已经呈现出独特的肠道微生物群谱，同时伴随着大脑多巴胺水平降低和棕色脂肪重塑紊乱。KSLP 治疗改善了宿主的健康状况，并通过增加厚壁菌门（Firmicutes）而减少拟杆菌门（Bacteroidetes）来调节肠道微生物群，特别是增加了与多巴胺和丁酸盐产生功能相关的细菌的相对丰度，这表明 KSLP 治疗构建了更健康的肠道环境。总之，通过肠道-大脑轴和肠道-代谢轴，肠道微生物群和丁酸盐的调节可能会连接性地调节多巴胺的产生和 BAT 的重塑。
