Food and Drug Safety Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Department of Biochemistry, Higher Education Institute of Rab-Rashid, Tabriz 51664, Iran.
Nutrition Research Center, Tabriz University of Medical Sciences, Tabriz, Iran; Biotechnology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Int J Biol Macromol. 2020 Jul 1;154:584-595. doi: 10.1016/j.ijbiomac.2020.03.093. Epub 2020 Mar 13.
Prostate-specific antigen (PSA) is a high molecular weight glycoprotein that is used as a marker for the diagnosis of prostate cancer and is therefore important in the medical field. In this study, a novel sandwich type immunoassay was designed based on encapsulation of biotinylated antibody into KCC-1-npr-NH. KCC-1-npr-NH stabilized the stability of the primary antibody. So, encapsulated Ab1 was immobilized on the surface of glassy carbon electrode. Field emission scanning electron microscope (FE-SEM) was employed to monitor the sensor fabrication. The engineered immunosensor was used for the detection of PSA using differential pulse voltammetry (DPVs) and square wave voltammetry (SWVs) techniques. The proposed interface lead to enhancement of accessible surface area for immobilizing a high amount of anti-PSA antibody, increasing electrical conductivity, boosting stability, catalytic properties and biocompatibility. The intensity of electrochemical signals is also increased by the use of AuNPs functionalized with CysA used in secondary antibody (HRP conjugated PSA) structure. Under optimal conditions, the designed immuno-assay provide a good analytical performance for quantifying the PSA marker in the linear range of 1 to 60 μg/l.
前列腺特异性抗原（PSA）是一种高分子量糖蛋白，被用作前列腺癌诊断的标志物，因此在医学领域非常重要。在本研究中，设计了一种基于将生物素化抗体包封入 KCC-1-npr-NH 中的新型夹心型免疫测定法。KCC-1-npr-NH 稳定了抗体的稳定性。因此，包封的 Ab1 被固定在玻碳电极的表面上。场发射扫描电子显微镜（FE-SEM）用于监测传感器的制造。该工程免疫传感器用于使用差分脉冲伏安法（DPVs）和方波伏安法（SWVs）技术检测 PSA。所提出的界面导致可用于固定大量抗 PSA 抗体的表面积增加，电导率增加，稳定性提高，催化性能和生物相容性提高。通过使用在二级抗体（HRP 缀合的 PSA）结构中使用的功能化有 CysA 的 AuNPs，电化学信号的强度也得到了增强。在最佳条件下，所设计的免疫分析方法在 1 至 60μg/l 的线性范围内提供了定量测定 PSA 标志物的良好分析性能。
