Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P ''1177'' 3018, Sfax, Tunisia; Departments of Life Sciences, Faculty of Sciences of Gafsa, Zarroug, 2112, Gafsa, Tunisia.
Biotechnology and Plant Improvement Laboratory, Centre of Biotechnology of Sfax (CBS)/University of Sfax, B.P ''1177'' 3018, Sfax, Tunisia.
Microb Pathog. 2020 Jun;143:104143. doi: 10.1016/j.micpath.2020.104143. Epub 2020 Mar 13.
The present study was undertaken to investigate the expression, purification and biological activities of a novel Triticum durum Annexin 12 protein (TdAnn12). The findings indicated that the molecular weight of the purified TdAnn12 was estimated to 35 kDa. The purified TdAnn12 protein was modulated by, Methyl-jasmonate, and ethephon treatments. The purified TdAnn12 protein displayed good antimicrobial activities against 9 tested pathogenic bacteria. The antioxidant activities showed that TdAnn12 displayed an excellent DPPH scavenging ability with an IC of 8.33 μg/ml and a strong Beta-carotene bleaching inhibition after 120 min of incubation with an IC of 2 μg/ml the cytotoxic effects of the TdAnn 12 showed that HepG2 and MCF-7 were examined by MTT assay. The IC values were 250.35 and 400.25 μg/ml for HepG2 and MCF-7 cells, respectively. The inhibitory effects of this TdAnn12 was assessed in vivo against Listeria monocytogenes, inoculated in minced beef meat at 6.10 CFU/g amended with different concentrations of the purified TdAnn12 and stored at 4 °C for 21 days. Results showed an excellent inhibitory effect of TdAnn12 of this pathogenic bacterium at 4 °C. Overall, the TdAnn12 have potential application as active ingredients in food and pharmaceutical industry.
本研究旨在研究一种新型的硬粒小麦 Annexin 12 蛋白（TdAnn12）的表达、纯化和生物学活性。研究结果表明，纯化的 TdAnn12 分子量估计为 35 kDa。经茉莉酸甲酯和乙烯利处理后，纯化的 TdAnn12 蛋白发生了变化。纯化的 TdAnn12 蛋白对 9 种测试的病原菌具有良好的抗菌活性。抗氧化活性表明，TdAnn12 对 DPPH 具有出色的清除能力，IC 为 8.33μg/ml，在 120 min 孵育后对β-胡萝卜素的漂白抑制作用较强，IC 为 2μg/ml。用 MTT 法检测 TdAnn12 对 HepG2 和 MCF-7 的细胞毒性作用。HepG2 和 MCF-7 细胞的 IC 值分别为 250.35 和 400.25μg/ml。在体内，用不同浓度的纯化 TdAnn12 处理并在 4°C 下储存 21 天，评估该 TdAnn12 对单核细胞增生李斯特菌的抑制作用。结果表明，该病原菌在 4°C 时对 TdAnn12 具有出色的抑制作用。总的来说，TdAnn12 有望作为食品和制药工业中的活性成分得到应用。
